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Whole‐Genome Regulation for Yeast Metabolic Engineering
Small Methods ( IF 12.4 ) Pub Date : 2019-11-18 , DOI: 10.1002/smtd.201900640
Shuangying Jiang 1 , Tong Si 1 , Junbiao Dai 1
Affiliation  

As the most thoroughly investigated eukaryotic microorganism, Saccharomyces cerevisiae is widely used as a cell factory for producing valuable compounds. Currently, strain construction and optimization still cost a vast amount of capital and time to achieve industrially relevant parameters. To efficiently excavate the genetic factors required for desired traits, many high‐throughput genome engineering tools are developed. This review focuses on the genome‐wide approaches that are applied in yeast. Through multiplex genome editing and/or transcription regulation, these tools generate cell populations with great genetic diversity, enabling identification of the critical factors and synergistic interactions in a short time.

中文翻译:

酵母代谢工程的全基因组法规

作为研究最深入的真核微生物,酿酒酵母被广泛用作生产有价值化合物的细胞工厂。当前,菌株的构建和优化仍然花费大量的资金和时间来获得工业上相关的参数。为了有效挖掘所需性状所需的遗传因素,开发了许多高通量基因组工程工具。这篇综述着重于酵母中应用的全基因组方法。通过多重基因组编辑和/或转录调控,这些工具可产生具有巨大遗传多样性的细胞群体,从而能够在短时间内识别关键因素和协同相互作用。
更新日期:2019-11-18
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