A MADS-domain transcription factorLoSVP, which could delay flowering through vernalization pathway, was isolated from lily. MADS-domain transcription factors play important roles in plant growth and development, especially in the transition from vegetative phase to reproductive phase. However, their functions in bulbous flowering plants are largely unknown. In this work, a SHORT VEGETATIVE PHASE (SVP) encoding genes LoSVP from oriental lily was isolated. Bioinformatic analyses demonstrated that LoSVP encodes a type II MADS-box protein containing a conserved MADS-box, as well as a conserved K-box domain. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed ubiquitous expression of LoSVP in various tissues, including petals, stamens, pistils, leaves and scales. Real-time polymerase chain reaction (PCR) analyses demonstrated that LoSVP was predominantly expressed in the early stage of developing flowers. Constitutive expression of LoSVP in Arabidopsis led to significantly delayed flowering of transgenic plants. These results suggest that LoSVP is involved in plant flowering and could be used as a potential candidate gene for the genetic regulation of flowering time in higher plants.

中文翻译：

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来自百合的 MADS 结构域转录因子 LoSVP 的异位表达导致转基因拟南芥开花延迟。

从百合中分离出一种可以通过春化途径延迟开花的MADS结构域转录因子LoSVP。MADS 结构域转录因子在植物生长发育中发挥重要作用，特别是在从营养阶段到生殖阶段的过渡中。然而，它们在球茎开花植物中的功能在很大程度上是未知的。在这项工作中，从东方百合中分离出一个编码基因LoSVP的短营养相（SVP）基因。生物信息学分析表明，LoSVP 编码一种 II 型 MADS-box 蛋白，其中包含一个保守的 MADS-box，以及一个保守的 K-box 结构域。半定量逆转录聚合酶链反应 (RT-PCR) 揭示了 LoSVP 在各种组织中的普遍表达，包括花瓣、雄蕊、雌蕊、叶​​子和鳞片。实时聚合酶链反应 (PCR) 分析表明，LoSVP 主要在开花的早期表达。LoSVP 在拟南芥中的组成型表达导致转基因植物的开花显着延迟。这些结果表明LoSVP参与植物开花，可作为高等植物开花时间遗传调控的潜在候选基因。