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Evaluation of interactive effects of UV light and nano encapsulation on the toxicity of azoxystrobin on zebrafish.
Nanotoxicology ( IF 5 ) Pub Date : 2019-11-18 , DOI: 10.1080/17435390.2019.1690064
Yueyang Zhang 1 , Claudia Sheedy 2 , Denise Nilsson 2 , Greg G Goss 1, 3, 4
Affiliation  

The use of nanotechnology to enhance pesticide formulations holds the promise of reduced pesticide use, reduced mobility in soils, and overall improvements in agricultural practices while simultaneously maintaining yields. However, the toxicity of nano-enabled pesticides, including azoxystrobin (Az), has not been well studied compared with their conventional form. This study investigates both lethal and sub-lethal endpoints in zebrafish embryos up to 120 h post-fertilization (hpf) under either laboratory light or simulated UV light. The median lethal concentration (LC50) value of nano-enabled Az (nAz) was significantly lower than the conventional form (Az). Interestingly, artificial UV light significantly increased toxicity (decreased LC50) of both Az and nAz. Malformations were not observed but the remaining yolk sac volume was significantly increased in both types of Az at both light conditions. This decreased yolk consumption is in agreement with reduced oxygen consumption and heart rate. Catalase enzyme activity was only reduced to UV light while superoxide dismutase activity was significantly reduced by co-exposure of UV light, and either type of Az at a nominal concentration of 100 μg L-1. The co-exposure of Az at 100 μg L-1 and UV light significantly upregulated sod1, sod2, and gpx1b expression and both types of Az significantly reduced gpx1a expression. Lipid peroxidation was significantly increased in nAz and Az at 100 μg L-1 under laboratory light, while UV light induced even higher level of lipid peroxidation. The results will provide important information on the toxicity of nAz under ecologically realistic conditions.

中文翻译:

评估紫外线和纳米封装对嘧菌酯对斑马鱼的毒性的相互作用的影响。

使用纳米技术增强农药配方有望减少农药的使用,减少土壤中的迁移率,并全面改善农业实践,同时保持产量。但是,与传统形式相比,纳米级农药(包括嘧菌酯(Az))的毒性尚未得到很好的研究。这项研究调查了受精后(hpf)达120 h的斑马鱼胚胎在实验室光或模拟UV光下的致死和次致死终点。具有纳米功能的Az(nAz)的中值致死浓度(LC50)值显着低于常规形式(Az)。有趣的是,人造紫外线明显增加了Az和nAz的毒性(降低了LC50)。没有观察到畸形,但是在两种光照条件下,两种类型的Az的剩余卵黄囊体积均显着增加。减少的蛋黄消耗量与减少的氧气消耗量和心率相吻合。过氧化氢酶的活性仅在紫外线下降低,而超氧化物歧化酶的活性通过紫外线和标称浓度为100μgL-1的两种Az的共同暴露而显着降低。在100μgL-1和紫外线下,Az的共同暴露显着上调了sod1,sod2和gpx1b的表达,两种类型的Az均显着降低了gpx1a的表达。在实验室光下,在100μgL-1下,nAz和Az中的脂质过氧化作用显着增加,而UV光诱导的脂质过氧化作用甚至更高。
更新日期:2019-11-18
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