An unclassified calicivirus (CV) detected in geese was recently reported and proposed as a new member of the family Caliciviridae. There is limited information about the epidemiology, etiology and detection method of goose-origin CV (GCV) to date. In this study, an EvaGreen based fluorescence quantitative real-time RT-PCR assay was developed and optimized for the detection of GCVs. The assay sensitively detected GCV RNA template with a good linear standard curve. We also demonstrated the specificity and reproducibility of the detection method for GCVs. Thus, the method developed in this study will benefit the investigation of possible sporadic outbreaks of CV infections in geese, as well as epidemiological and etiological studies of GCVs.

中文翻译：

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基于EvaGreen的实时RT-PCR分析方法的开发，用于快速检测，定量和诊断鹅杯状病毒。

最近报道了在鹅中检测到的未分类杯状病毒（CV），并提出将其作为杯状病毒科的新成员。迄今为止，关于鹅源CV（GCV）的流行病学，病因学和检测方法的信息有限。在这项研究中，开发了基于EvaGreen的荧光定量实时RT-PCR测定法，并对其进行了优化以检测GCV。该测定法灵敏地检测到具有良好线性标准曲线的GCV RNA模板。我们还证明了GCV检测方法的特异性和可重复性。因此，本研究中开发的方法将有助于调查鹅中CV感染可能的零星爆发以及GCV的流行病学和病因学研究。