Application of polyester-degrading enzymes should be considered as an eco-friendly alternative to chemical recycling due to the huge plastic waste disposal nowadays. Many hydrolases from several fungi and bacteria have been discovered and successfully evaluated for their activity towards different aliphatic polyesters (PHA, PBS, PBSA, PCL, PLA), aromatic polyesters (PET, PBT, PMT) as well as their co-polyesters (PBST, PBAT, PBSTIL). This revision gives an up-to-date overview on the main biochemical features and biotechnological applications of those reported enzymes which are able to degrade polyester-based plastics, including different microbial polyester depolymerases, esterases, cutinase-like enzymes and lipases. Summarized information includes available protein sequences with the corresponding accession numbers deposited in NCBI server, 3D resolved structures, and data about optimal conditions for enzymatic activity and stability of many of these microbial enzymes that would be helpful for researchers in this topic. Although screening and identification of new native polyester hydrolases from microbial sources is undeniable according to literature, we briefly highlight the importance of the design of improved enzymes towards recalcitrant aromatic polyesters through different approaches that include site-directed mutagenesis and surface protein engineering.

中文翻译：

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微生物酶的生物化学特性和生物技术应用涉及聚酯型塑料的降解。

由于当今处理大量的塑料废物，应考虑使用降解聚酯的酶作为化学回收的一种环保替代方法。已经发现了来自多种真菌和细菌的许多水解酶，并成功评估了它们对不同脂族聚酯（PHA，PBS，PBSA，PCL，PLA），芳族聚酯（PET，PBT，PMT）及其共聚酯（PBST）的活性。 ，PBAT，PBSTIL）。该修订版对那些已报道的能够降解基于聚酯的塑料的酶的主要生化特征和生物技术应用进行了最新概述，这些酶包括不同的微生物聚酯解聚酶，酯酶，角质酶样酶和脂肪酶。汇总信息包括可用的蛋白质序列，其相应的登录号已存储在NCBI服务器中，3D解析的结构以及有关许多此类微生物酶的酶活性和稳定性的最佳条件的数据，这将对本主题的研究人员有所帮助。尽管从文献中筛选和鉴定新的天然聚酯水解酶是不可否认的，但我们简要强调了通过包括定点诱变和表面蛋白工程在内的不同方法设计改良的酶对难降解的芳香族聚酯的重要性。