Insights into the structure and dynamics of large biological systems has been greatly improved by two concurrent NMR approaches: the application of transverse relaxation-optimized spectroscopy (TROSY) techniques in multi-dimensional NMR, especially the methyl-TROSY, and the resurgence of ¹⁹F NMR using trifluoromethyl (CF₃) probes. Herein we investigate the feasibility of combining these approaches into a trifluoromethyl-TROSY experiment. Using a CF₃-labelled parvalbumin, we have evaluated the natural abundance ¹³C-¹⁹F correlation spectra and find no indication of a CF₃ TROSY at high magnetic fields.

中文翻译：

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高磁场下三氟甲基TROSY NMR的可行性

两种同时进行的NMR方法大大改善了对大型生物系统的结构和动力学的洞察力：横向弛豫优化光谱（TROSY）技术在多维NMR中的应用，尤其是甲基TROSY，以及¹⁹ F的复活使用三氟甲基（CF ₃）探针的NMR 。本文中，我们研究了将这些方法结合到三氟甲基-TROSY实验中的可行性。使用CF ₃标记的小白蛋白，我们评估了自然丰度¹³ C- ¹⁹ F相关光谱，在强磁场下未发现CF ₃ TROSY的迹象。