Cystic echinococcosis is a chronic and complex zoonotic disease. The mechanisms underlying the parasite's establishment, growth and persistence are not completely understood, and are thought be modulated by a crosstalk through extracellular vesicles. Here, EVs were isolated from the hydatid cyst fluid of patients with cystic echinococcosis and protoscolex culture supernatant. Proteomic analysis of these EVs revealed several parasite- and human-derived proteins. Very few studies have performed proteomic analysis of EVs isolated from HCF and PCS. Our proteomic analysis of the EVs derived from HCF and PCS facilitated identification of 1175 proteins, wherein 1026 and 38 proteins were exclusively identified in the EVs derived from HCF (HCF-EVs) and PCS (PCS-EVs), respectively, and 111 proteins were shared in both. The results of co-culture of PCS-EVs with murine peripheral blood mononuclear cells showed that PCS-EVs significantly regulated T lymphocyte functions in a dose-dependent manner. Collectively, our results provide valuable information on parasite survival strategies and new insights into the role of these EVs in the establishment and persistence of hydatid cysts.

中文翻译：

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来自患者的棘球棘球d囊液的细胞外囊泡：T细胞免疫调节功能的分离，鉴定和评估。

囊性棘球co病是一种慢性和复杂的人畜共患病。寄生虫的建立，生长和持久性的潜在机制尚不完全清楚，并且认为是通过细胞外小泡的串扰来调节的。在这里，从患有囊性棘球cc虫病和原粘菌培养上清液的患者的包虫囊液中分离出EV。这些电动汽车的蛋白质组学分析揭示了几种寄生虫和人类衍生的蛋白质。很少有研究对从HCF和PCS分离出的EV进行蛋白质组学分析。我们对HCF和PCS衍生的EV的蛋白质组学分析有助于鉴定1175种蛋白质，其中1026和38种蛋白质分别在HCF（HCF-EV）和PCS（PCS-EV）衍生的EV中鉴定，其中111种蛋白质分别为两者共享。PCS-EVs与鼠外周血单个核细胞共培养的结果表明，PCS-EVs以剂量依赖的方式显着调节T淋巴细胞的功能。总的来说，我们的结果为寄生虫生存策略提供了有价值的信息，并为这些电动车在包虫囊肿的建立和持续中的作用提供了新的见解。