Antibiotic and antibiotic resistance genes (ARGs) have frequently detected in the effluent from wastewater treatment plants. Biological activated carbon (BAC) filter as a tertiary process was used to study the influence of tetracycline (TC) on nitrogen removal efficiency during exposure to tetracycline at different concentrations (0, 0.01, 0.1, 1, 5 mg/L) in an easily separated biofilm (interstitial organism) and inner biofilm (carbon-attached organism). High throughput sequencing and QPCR showed that exposure of 5 mg/L tetracycline increased the denitrifying genes (DNGs) and genera containing denitrification function (e.g., Acidovorax, Flavobacterium, unclassified_f_Comamonadaceae). Moreover, DNGs were mainly located in carbon-attached organisms, while the resistance genes were primarily found in interstitial organisms. The presence of TC stimulated the generation of resistance genes and DNGs, leading to enhanced nitrogen removal. Approximately 90% of the total resistance genes in interstitial organisms and 85% of the TC were effectively removed in the developed biofilm system. Overall, the results indicated that the biofilm system was a good choice for treatment of sewage treatment with a certain amount of TC and control over the contamination of resistance genes.

废水处理厂的废水中经常检测到抗生素和抗生素抗性基因（ARG）。使用生物活性炭（BAC）过滤器作为第三级工艺来研究四环素（TC）在以不同浓度（0、0.01、0.1、1、5 mg / L）轻松暴露于四环素的过程中对脱氮效率的影响。分离生物膜（间质生物）和内部生物膜（碳附着生物）。高通量测序和QPCR显示，暴露于5 mg / L四环素会增加反硝化基因（DNG）和含有反硝化功能的属（例如，嗜酸杆菌属，黄杆菌属，未分类。此外，DNGs主要位于附着碳的生物中，而抗性基因则主要存在于间质生物中。TC的存在刺激了抗性基因和DNG的产生，从而提高了氮的去除率。在已开发的生物膜系统中，间质生物中约90％的总抗性基因和85％的TC被有效去除。总体而言，结果表明，生物膜系统是用于处理含一定量TC和控制抗性基因污染的污水处理的理想选择。