Gut-dwelling Prevotella copri (P. copri), the most prevalent Prevotella species in the human gut, have been associated with diet and disease. However, our understanding of their diversity and function remains rudimentary because studies have been limited to 16S and metagenomic surveys and experiments using a single type strain. Here, we describe the genomic diversity of 83 P. copri isolates from 11 human donors. We demonstrate that genomically distinct isolates, which can be categorized into different P. copri complex clades, utilize defined sets of polysaccharides. These differences are exemplified by variations in susC genes involved in polysaccharide transport as well as polysaccharide utilization loci (PULs) that were predicted in part from genomic and metagenomic data. Functional validation of these PULs showed that P. copri isolates utilize distinct sets of polysaccharides from dietary plant, but not animal, sources. These findings reveal both genomic and functional differences in polysaccharide utilization across human intestinal P. copri strains.

中文翻译：

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人类肠道普氏菌分离物的不同多糖利用概况。

居于肠道的普氏菌属 (P. copri) 是人类肠道中最普遍的普氏菌属物种，与饮食和疾病有关。然而，我们对其多样性和功能的理解仍然是初步的，因为研究仅限于 16S 和宏基因组调查以及使用单一类型菌株的实验。在这里，我们描述了来自 11 个人类供体的 83 个 P. copri 分离株的基因组多样性。我们证明了基因组上不同的分离物，可分为不同的 P. copri 复合进化枝，利用定义的多糖集。这些差异的例证是参与多糖转运的 susC 基因的变异以及多糖利用基因座 (PUL) 的变异，这些变异部分是从基因组和宏基因组数据中预测的。这些 PUL 的功能验证表明 P. copri 分离物利用来自膳食植物而非动物来源的不同多糖组。这些发现揭示了人类肠道 P. copri 菌株中多糖利用的基因组和功能差异。