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Simultaneous encapsulation of hydrophilic and lipophilic molecules in liposomes of DSPC
Thermochimica Acta ( IF 3.5 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.tca.2019.178462 Mariana R. Romero-Arrieta , Elizabeth Uria-Canseco , Silvia Perez-Casas
Thermochimica Acta ( IF 3.5 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.tca.2019.178462 Mariana R. Romero-Arrieta , Elizabeth Uria-Canseco , Silvia Perez-Casas
Abstract This study shows simultaneous incorporation of two hydrophobic molecules and a hydrophilic molecule into distearoylphosphatidylcholine (DSPC) liposomes. Pinocembrin and cholesterol are incorporated in the lipidic membrane while resazurin is encapsulated in the aqueous center. The thermotropic behavior of liposomes as a function of the additives was studied with differential scanning calorimetry and the morphology was observed by SEM. A high percentage of entrapment was found for different concentrations of pinocembrin. Cholesterol does not interfere with the incorporation efficiency of pinocembrin and improves the distribution in the membrane but decreases the dissolution stability of liposomes due to steric hindrance in the bilayer. Encapsulation efficiency of resazurin was close to 50%. This molecule also interacts with the polar heads of the phospholipids. This research offers an alternative formulation by using DSPC liposomes as carrier of molecules with different polarity to evaluate the cytotoxicity of pinocembrin in cancer cell lines.
中文翻译:
在DSPC的脂质体中同时包封亲水性和亲脂性分子
摘要 本研究表明两个疏水分子和一个亲水分子同时掺入二硬脂酰磷脂酰胆碱 (DSPC) 脂质体中。Pinocembrin 和胆固醇结合在脂质膜中,而刃天青则包裹在水性中心。用差示扫描量热法研究了脂质体的热致行为作为添加剂的函数,并通过 SEM 观察形态。发现不同浓度的松香素有很高的截留率。胆固醇不会干扰松香素的掺入效率并改善膜中的分布,但由于双层中的空间位阻而降低脂质体的溶解稳定性。刃天青的包封率接近50%。该分子还与磷脂的极性头相互作用。该研究提供了一种替代配方,通过使用 DSPC 脂质体作为不同极性分子的载体来评估松树素在癌细胞系中的细胞毒性。
更新日期:2020-05-01
中文翻译:
在DSPC的脂质体中同时包封亲水性和亲脂性分子
摘要 本研究表明两个疏水分子和一个亲水分子同时掺入二硬脂酰磷脂酰胆碱 (DSPC) 脂质体中。Pinocembrin 和胆固醇结合在脂质膜中,而刃天青则包裹在水性中心。用差示扫描量热法研究了脂质体的热致行为作为添加剂的函数,并通过 SEM 观察形态。发现不同浓度的松香素有很高的截留率。胆固醇不会干扰松香素的掺入效率并改善膜中的分布,但由于双层中的空间位阻而降低脂质体的溶解稳定性。刃天青的包封率接近50%。该分子还与磷脂的极性头相互作用。该研究提供了一种替代配方,通过使用 DSPC 脂质体作为不同极性分子的载体来评估松树素在癌细胞系中的细胞毒性。