Salinity-induced metabolic, ionic, and transcript modifications in plants have routinely been studied using whole plant tissues, which do not provide information on spatial tissue responses. The aim of this study was to assess the changes in the lipid profiles in a spatial manner and to quantify the changes in the elemental composition in roots of seedlings of four barley cultivars before and after a short-term salt stress. We used a combination of liquid chromatography-tandem mass spectrometry, inductively coupled plasma mass spectrometry, matrix-assisted laser desorption/ionization mass spectrometry imaging, and reverse transcription - quantitative real time polymerase chain reaction platforms to examine the molecular signatures of lipids, ions, and transcripts in three anatomically different seminal root tissues before and after salt stress. We found significant changes to the levels of major lipid classes including a decrease in the levels of lysoglycerophospholipids, ceramides, and hexosylceramides and an increase in the levels of glycerophospholipids, hydroxylated ceramides, and hexosylceramides. Our results revealed that modifications to lipid and transcript profiles in plant roots in response to a short-term salt stress may involve recycling of major lipid species, such as phosphatidylcholine, via resynthesis from glycerophosphocholine.

中文翻译：

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比较空间脂质组学分析显示，响应盐度，大麦根不同发育区的细胞脂质重塑。

盐度诱导的代谢，离子和转录物修饰在植物中已常规使用整株植物组织进行了研究，但没有提供有关空间组织反应的信息。这项研究的目的是以空间方式评估脂质分布的变化，并量化短期盐胁迫前后四个大麦品种幼苗根中元素组成的变化。我们结合使用了液相色谱串联质谱，电感耦合等离子体质谱，基质辅助激光解吸/电离质谱成像和逆转录-定量实时聚合酶链反应平台，以检查脂质，离子，盐胁迫前后在三个解剖学上不同的精根组织中的转录本和转录本 我们发现主要脂质类别的水平发生了重大变化，包括溶血甘油磷脂，神经酰胺和己糖基神经酰胺的水平降低，以及甘油磷脂，羟基化神经酰胺和己糖基神经酰胺的水平增加。我们的结果表明，响应短期盐胁迫而对植物根部脂质和转录物谱的修饰可能涉及通过从甘油磷酸胆碱中重新合成来回收主要脂质种类，例如磷脂酰胆碱。