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Programming Accessibility of DNA Monolayers for Degradation-Free Whole-Blood Biosensors
ACS Materials Letters ( IF 11.4 ) Pub Date : 2019-11-13 , DOI: 10.1021/acsmaterialslett.9b00404
Mengying Deng 1, 2, 3 , Min Li 3 , Fan Li 3 , Xiuhai Mao 3 , Qian Li 3 , Jianlei Shen 3 , Chunhai Fan 3 , Xiaolei Zuo 3
Affiliation  

Biosensors for direct detection of circulating biomarkers are of great importance for clinical diagnosis. Nevertheless, preservation of biomolecular probes in the recognition layer of biosensors remains a high challenge in such complex matrices (e.g., whole blood). Here we report the development of a nuclease-resistant DNA monolayer for degradation-free biosensing in whole blood. The accessibility of surface-confined DNA monolayers was programmed by engineering the lateral distances of DNA probes. In particular, we found that nucleases were effectively repelled from the biosensor surface by tuning the DNA density to 25.5 × 1012 molecules/cm2, equivalent to an interstrand lateral distance of ∼2 nm. As a proof-of-concept, we fabricated an electrochemical DNA aptamer sensor for detecting a small-molecule drug doxorubicin (Dox) that exhibited excellent stability and accuracy when deployed for monitoring Dox levels in whole blood. We thus expect the accessibility-programmable DNA biosensors to hold great potential for on-site biomarker analysis and drug monitoring.

中文翻译:

用于无降解全血生物传感器的DNA单层的编程可访问性

直接检测循环生物标志物的生物传感器对于临床诊断非常重要。然而,在这种复杂的基质(例如全血)中,将生物分子探针保存在生物传感器的识别层中仍然是一个很高的挑战。在这里,我们报告了全血中无降解生物传感的耐核酸酶DNA单层的发展。通过设计DNA探针的横向距离,可以对表面受限的DNA单层的可访问性进行编程。特别是,我们发现通过将DNA密度调整为25.5×10 12分子/ cm 2可以有效地从生物传感器表面排斥核酸酶。,等于约2 nm的链间横向距离。作为概念验证,我们制造了一种电化学DNA适体传感器,用于检测小分子药物阿霉素(Dox),该传感器在部署用于监测全血中Dox水平时表现出出色的稳定性和准确性。因此,我们希望可访问性可编程的DNA生物传感器在现场生物标志物分析和药物监测方面具有巨大的潜力。
更新日期:2019-11-13
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