We show that a multi-selective excitation with Hadamard encoding is a powerful tool for 2D acquisition of 13 C-13 C homo-nuclear correlations. This method is not designed to improve the sensitivity, but rather to reduce the experiment time, provided there is sufficient sensitivity. Therefore, it allows fast acquisition of such 2D spectra in labeled molecules. The technique has been demonstrated using a U-13 C-15 N histidine hydrochloride monohydrate sample allowing each point of the build-up curves of the 13 C-13 C cross-peaks to be recorded within 4 min 35 s, which is very difficult with conventional methods. Using the U-13 C-15 N f-MLF sample we have demonstrated that the method can be applied to molecules with fourteen 13 C resonances with a minimum frequency separation of 240 Hz.

中文翻译：

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Hadamard 获取 13 C-13 C 2D 相关核磁共振谱

我们表明使用 Hadamard 编码的多选择性激发是 13 C-13 C 同核相关性 2D 采集的强大工具。这种方法不是为了提高灵敏度，而是为了减少实验时间，前提是有足够的灵敏度。因此，它允许快速获取标记分子中的此类 2D 光谱。该技术已使用 U-13 C-15 N 盐酸组氨酸盐酸盐一水合物样品进行了证明，允许在 4 分 35 秒内记录 13 C-13 C 交叉峰的累积曲线的每个点，这非常困难用常规方法。使用 U-13 C-15 N f-MLF 样品，我们已经证明该方法可以应用于具有十四个 13 C 共振的分子，最小频率间隔为 240 Hz。