当前位置: X-MOL 学术Microb. Biotechnol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Diamondoids are not forever: microbial biotransformation of diamondoid carboxylic acids.
Microbial Biotechnology ( IF 5.7 ) Pub Date : 2019-11-12 , DOI: 10.1111/1751-7915.13500
Benjamin D Folwell 1 , Terry J McGenity 1 , Corinne Whitby 1
Affiliation  

Oil sands process‐affected waters (OSPW) contain persistent, toxic naphthenic acids (NAs), including the abundant yet little‐studied diamondoid carboxylic acids. Therefore, we investigated the aerobic microbial biotransformation of two of the most abundant, chronically toxic and environmentally relevant diamondoid carboxylic acids: adamantane‐1‐carboxylic acid (A1CA) and 3‐ethyl adamantane carboxylic acid (3EA). We inoculated into minimal salts media with diamondoid carboxylic acids as sole carbon and energy source two samples: (i) a surface water sample (designated TPW) collected from a test pit from the Mildred Lake Settling Basin and (ii) a water sample (designated 2 m) collected at a water depth of 2 m from a tailings pond. By day 33, in TPW enrichments, 71% of A1CA and 50% of 3EA was transformed, with 50% reduction in EC20 toxicity. Similar results were found for 2 m enrichments. Biotransformation of A1CA and 3EA resulted in the production of two metabolites, tentatively identified as 2‐hydroxyadamantane‐1‐carboxylic acid and 3‐ethyladamantane‐2‐ol respectively. Accumulation of both metabolites was less than the loss of the parent compound, indicating that they would have continued to be transformed beyond 33 days and not accumulate as dead‐end metabolites. There were shifts in bacterial community composition during biotransformation, with Pseudomonas species, especially P. stutzeri, dominating enrichments irrespective of the diamondoid carboxylic acid. In conclusion, we demonstrated the microbial biotransformation of two diamondoid carboxylic acids, which has potential application for their removal and detoxification from vast OSPW that are a major environmental threat.

中文翻译:

类固醇不是永远存在的:类固醇羧酸的微生物生物转化。

受油砂加工影响的水(OSPW)包含持久的有毒环烷酸(NAs),包括数量丰富但研究较少的类金刚石羧酸。因此,我们研究了两种最丰富的,慢性毒性和与环境相关的类金刚石羧酸的需氧微生物生物转化:金刚烷-1-羧酸(A1CA)和3-乙基金刚烷羧酸(3EA)。我们将含有类金刚石羧酸作为唯一碳和能源的最小盐介质接种到两个样品中:(i)从米尔德雷德湖沉淀池的测试坑中收集的地表水样品(指定为TPW)和(ii)水样品(指定为2 m)从尾矿池中以2 m的水深收集。到第33天,在TPW浓缩中,转化了71%的A1CA和50%的3EA,其中EC降低了50%20毒。对于2 m富集,发现了相似的结果。A1CA和3EA的生物转化产生了两种代谢产物,分别确定为2-羟基金刚烷-1-羧酸和3-乙基金刚烷-2-醇。两种代谢物的积累都少于母体化合物的损失,这表明它们将继续转化超过33天,并且不会以死胡同代谢物的形式积累。在生物转化过程中,细菌群落组成发生了变化,其中假单胞菌属尤其是斯图氏假单胞菌,不管类金刚石羧酸如何,主要的富集。总而言之,我们证明了两种类金刚石羧酸的微生物生物转化,这对于将其从巨大的OSPW中去除和排毒具有潜在的应用潜力,而OSPW是对环境的重大威胁。
更新日期:2019-11-12
down
wechat
bug