Abstract A simple, sensitive, and selective fluorimetric method is reported for the determination of β-carotene in various food samples. Three fluorescent dyes, including fluorescein (F), eosin B (EB), and Congo red (CR), were characterized in a series of measurements to optimize the analytical response for the reliable, suitable, and sustainable determination of β-carotene. 3′,6′-Dihydroxyspiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, commonly known as fluorescein, was selected for the fluorimetric determination of β-carotene using a phosphate buffer. Under the optimum experimental conditions, β-carotene was determined across a range from 0.54 to 536.87 mg L−1 with a detection limit equal to 0.47 mg L−1. The novel method was demonstrated to be easy to use, cost effective, rapid, and less complex compared to a standard protocol that involves a time-consuming extraction followed by an expensive HPLC determination.

中文翻译：

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使用荧光染料荧光测定食品样品中的 β-胡萝卜素

摘要 本文报道了一种简单、灵敏、选择性强的荧光法测定各种食品样品中的β-胡萝卜素。三种荧光染料，包括荧光素 (F)、曙红 B (EB) 和刚果红 (CR)，在一系列测量中进行了表征，以优化分析响应，从而可靠、合适和可持续地测定 β-胡萝卜素。选择 3',6'-Dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one，通常称为荧光素，用于使用磷酸盐缓冲液对 β-胡萝卜素进行荧光测定。在最佳实验条件下，β-胡萝卜素的测定范围为 0.54 至 536.87 mg L-1，检测限为 0.47 mg L-1。这种新方法被证明易于使用、经济高效、快速、