Upconverting nanoparticles are a rising class of non-linear luminescent probes burgeoning since the beginning of the 2000’s, especially for their attractiveness in theranostics. However, the precise quantification of the light delivered remains a hot problem in order to estimate their impact on the biological medium. Sophisticated photophysical measurements under near infrared excitation have been developed only by few teams. Here, we present the first attempt towards a simple and cheap photochemical approach consisting of an actinometric characterization of the green emission of NaYF₄:Yb,Er nanoparticles. Using the recently calibrated actinometer 1,2-bis(2,4-dimethyl-5-phenyl-3-thienyl)-3,3,4,4,5,5-hexafluoro-1-cyclopentene operating in the green region of the visible spectra, we propose a simple photochemical experiment to get an accurate estimation of the efficiency of these green-emitting “nanolamps”. The agreement of the collected data with the previous published results validates this approach.

中文翻译：

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光化学法测定上转换纳米粒子的发光效率

自2000年代初以来，上转换的纳米粒子是一类新兴的非线性发光探针，尤其是由于它们在治疗学上的吸引力而迅速兴起。然而，为了估计其对生物介质的影响，精确定量传递的光仍然是一个热门问题。仅少数团队开发了在近红外激发下进行复杂的光物理测量。在这里，我们提出了一种简单而廉价的光化学方法的首次尝试，该方法包括对NaYF ₄绿色发射的光化学计量学表征。：Yb，Er纳米粒子 使用最近校准的光度计，在绿色区域操作的1,2-双（2,4-二甲基-5-苯基-3-噻吩基）-3,3,4,4,5,5-六氟-1-环戊烯可见光谱，我们提出了一个简单的光化学实验，以准确估计这些绿色发光“ nanolamps”的效率。收集的数据与先前发布的结果的一致性验证了该方法。