This study investigated the biocontrol efficiency of Metschnikowia citriensis strain FL01 against Geotrichum citri-aurantii, and evaluated possible mechanisms. The results showed that M. citriensis could effectively control the development of sour rot, and significantly inhibit the mycelial growth and spore germination of G. citri-aurantii. The population dynamics results and Scanning electron microscopy (SEM) analysis indicated that M. citriensis could rapidly colonize wounds and tightly adhere to the surface of the wounds to compete with G. citri-aurantii for nutrition and space. M. citriensis also showed the biofilm formation action in vitro. The response of G. citri-aurantii to different components of M. citriensis culture showed that only the yeast cells but not the extracellular metabolites and the volatile organic compounds (VOCs) exhibited inhibitory effect on the growth of G. citri-aurantii. M. citriensis adhered to the hyphae of G. citri-aurantii loosely and sparsely, and the production of lytic enzymes β-1, 3-glucanase (GLU) and Chitinase (CHI) could not be induced by G. citri-auranti. Iron affected the pulcherrimin pigment production and antagonism of M. citriensis indicating iron depletion as the most important antagonistic mechanism. Besides, M. citriensis also induced resistance of fruit against sour rot. These results suggested that M. citriensis could be used as the potential alternative of fungicides to control postharvest pathogens on citrus fruit.

中文翻译：

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桔小球菌对柑桔土引起采后柑桔腐烂的生防能力和作用机理。

这项研究调查了Metschnikowia citriensis菌株FL01对Gitrichum citri-aurantii的生物防治效率，并评估了可能的机制。结果表明，柠檬黄单胞菌能有效控制酸腐的发生，并显着抑制柠檬黄单胞菌的菌丝生长和孢子萌发。种群动态结果和扫描电子显微镜（SEM）分析表明，柠檬分枝杆菌可以迅速定居在伤口上，并紧密附着在伤口表面，从而与柠檬分枝杆菌竞争营养和空间。柠檬曲霉还显示了体外的生物膜形成作用。柠檬绿假单胞菌对M.的不同成分的响应 柠檬文化表明只有酵母细胞而不对胞外代谢产物和挥发性有机化合物（VOCs）表现出抑制作用。柠檬曲霉松散地和稀疏地附着在柠檬曲霉的菌丝上，而柠檬曲霉不能诱导裂解酶β-1、3-葡聚糖酶（GLU）和几丁质酶（CHI）的产生。铁影响了桔梗中pulcherrimin色素的产生和拮抗作用，表明铁耗竭是最重要的拮抗机制。此外，柠檬分枝杆菌还诱导了果实对酸腐的抗性。这些结果表明，柑桔分枝杆菌可以用作控制柑橘类水果收获后病原体的杀真菌剂的潜在替代品。citriensis松散地和稀疏地附着在citri-aurantii的菌丝上，而citri-auranti不能诱导裂解酶β-1、3-葡聚糖酶（GLU）和几丁质酶（CHI）的产生。铁影响了桔梗中pulcherrimin色素的产生和拮抗作用，表明铁耗竭是最重要的拮抗机制。此外，柠檬分枝杆菌还诱导了果实对酸腐的抗性。这些结果表明，柑桔分枝杆菌可以用作控制柑橘类水果收获后病原体的杀真菌剂的潜在替代品。citriensis松散地和稀疏地附着在citri-aurantii的菌丝上，而citri-auranti不能诱导裂解酶β-1、3-葡聚糖酶（GLU）和几丁质酶（CHI）的产生。铁影响了桔梗中pulcherrimin色素的产生和拮抗作用，表明铁耗竭是最重要的拮抗机制。此外，柠檬分枝杆菌还诱导了果实对酸腐的抗性。这些结果表明，柑桔分枝杆菌可以用作控制柑橘类水果收获后病原体的杀真菌剂的潜在替代品。柠檬铁表明铁耗竭是最重要的拮抗机制。此外，柠檬分枝杆菌还诱导了果实对酸腐的抗性。这些结果表明，柑桔分枝杆菌可以用作控制柑橘类水果收获后病原体的杀真菌剂的潜在替代品。柠檬铁表明铁耗竭是最重要的拮抗机制。此外，柠檬分枝杆菌还诱导了果实对酸腐的抗性。这些结果表明，柑桔分枝杆菌可以用作控制柑橘类水果收获后病原体的杀真菌剂的潜在替代品。