We aimed to evaluate serum vaspin and its gene expression in patients with type 2 diabetes mellitus (T2DM) and to assess the association of serum vaspin and its gene expression with susceptibility of ischemic stroke (IS). The prospective case‐control study included 50 healthy individuals in a control group, and 90 patients with and T2DM were stratified into two subgroups: patients with IS and patients without IS. The serum vaspin concentration was measured by enzyme‐linked immunosorbent assay (ELISA). Real‐time PCR was performed to detect the mRNA expression of vaspin. Serum vaspin and vaspin expression levels were significantly higher in IS compared to the non‐IS group. Interestingly, they were positively correlated with other vascular and metabolic risks. Diastolic and systolic blood pressure, as well as hemoglobin A1c cholesterol (HbA1c), were independently correlated with serum vaspin. After adjusting for the traditional risk factors, the logistic regression analysis test was done to evaluate the predictor of IS among T2DM patients; the vaspin expression level was a statistical significance predictor of IS among T2DM patients. In conclusion, the higher levels of serum vaspin and vaspin expression levels in T2DM emphasizes the pivotal role of vaspin serum level and expression in the progression of metabolic and glucose abnormalities, thus, they could be used as biomarkers of IS.

中文翻译：

---

vaspin基因表达及其血清水平与2型糖尿病埃及患者缺血性卒中风险的关联：前瞻性病例对照研究。

我们旨在评估2型糖尿病（T2DM）患者的血清vaspin及其基因表达，并评估血清vaspin及其基因表达与缺血性中风（IS）的相关性。这项前瞻性病例对照研究包括了对照组中的50名健康个体，并将90例患有T2DM和T2DM的患者分为两个亚组：IS患者和非IS患者。血清vaspin浓度通过酶联免疫吸附测定（ELISA）进行测量。进行实时PCR以检测vaspin的mRNA表达。与非IS组相比，IS中的血清vaspin和vaspin表达水平显着更高。有趣的是，它们与其他血管和代谢风险呈正相关。舒张压和收缩压以及血红蛋白A1c胆固醇（HbA1c），与血清vaspin独立相关。在调整了传统的危险因素后，进行了logistic回归分析测试，以评估T2DM患者中IS的预测因素。vaspin表达水平是T2DM患者中IS的统计学显着性预测指标。总之，T2DM中较高的血清vaspin和vaspin表达水平强调了vaspin血清水平和表达在代谢和葡萄糖异常进展中的关键作用，因此，它们可用作IS的生物标记。