Mast cells are generally classified into two phenotypically distinct populations: mucosal-type mast cells (MMCs) and connective tissue-type mast cells (CTMCs). However, the molecular basis determining the different characteristics of the mast cell subclasses still remains unclear. Unfortunately, the number of mast cells that can be obtained from tissues is limited, which makes it difficult to study the function of each mast cell subclass. Here, we report the generation and characterization of MMCs and CTMCs derived from mouse BM mast cells (BMMCs). We found that the expression of galanin receptor 3 was elevated in MMCs when compared to the expression in CTMCs. Moreover, intraperitoneal injection of a galanin antagonist reduced MMCs and inhibited the inflammation of dextran sodium sulfate-induced colitis in mice. Therefore, these results suggest that galanin promotes MMC differentiation in vivo, and provide important insights into the molecular mechanisms underlying the differentiation of mast cell subclasses.

中文翻译：

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甘丙肽在小鼠粘膜肥大细胞分化中的作用。

肥大细胞通常分为两个表型不同的种群：粘膜型肥大细胞（MMC）和结缔组织型肥大细胞（CTMC）。然而，确定肥大细胞亚类的不同特征的分子基础仍然不清楚。不幸的是，可以从组织获得的肥大细胞的数量有限，这使得难以研究每个肥大细胞亚类的功能。在这里，我们报告的生成和表征从小鼠BM肥大细胞（BMMCs）的MMC和CTMCs。我们发现与CTMC中的表达相比，MMC中的甘丙肽受体3的表达升高。此外，腹膜内注射甘丙肽拮抗剂减少了小鼠的MMC，并抑制了葡聚糖硫酸钠诱导的小鼠结肠炎的炎症。所以，