Redox processes regulate plant/insect responses, but the precise roles of environmental triggers and specific molecular components remain poorly defined. Aphid fecundity and plant responses were therefore measured in Arabidopsis thaliana mutants deficient in either catalase 2 (cat2), different protein phosphatase 2A (PP2A) subunits or glutathione (cad2, pad2, and clt1) under either moderate (250 μmol m-2 s-1 ) or high (800 μmol m-2 s-1 ) light. Aphid fecundity was decreased in pp2a-b'γ, cat2 and the cat2 pp2a-b'γ double mutants relative to the wild type under moderate irradiance. High light decreased aphid numbers in all genotypes except for cat2. Aphid fecundity was similar in the cat2 and glutathione-, phytoalexin-, and glucosinolate-deficient cat2cad2 double mutants under both irradiances. Aphid-induced increases in transcripts encoding the abscisic acid-related ARABIDOPSIS ZINC-FINGER PROTEIN 1 transcription factor were observed only under moderate light. Conversely, aphid induced increases in transcripts encoding the jasmonate-synthesis enzyme ALLENE OXIDE CYCLASE 3 was observed in all genotypes only under high light. Aphid-induced increases in REDOX RESPONSIVE TRANSCRIPTION FACTOR 1 mRNAs were observed in all genotypes except pp2a-b'ζ1-1 under both irradiances. Aphid fecundity is therefore regulated by cellular redox signalling that is mediated, at least in part, through PP2A-dependent mitochondria to nucleus signalling pathways.

中文翻译：

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过氧化氢酶，谷胱甘肽和蛋白磷酸酶2A依赖的细胞器氧化还原信号在中等和高辐照度下调节蚜虫的繁殖力。

氧化还原过程调节植物/昆虫的反应，但对环境触发因素和特定分子成分的确切作用仍知之甚少。因此，在中度（250μmolm-2 s-s）下，在缺乏过氧化氢酶2（cat2），不同蛋白磷酸酶2A（PP2A）亚基或谷胱甘肽（cad2，pad2和clt1）的拟南芥突变体中，测定了蚜虫的繁殖力和植物应答。 1）或高（800μmolm-2 s-1）的光。在中等辐照度下，相对于野生型，pp2a-b'γ，cat2和cat2pp2a-b'γ双突变体的蚜虫繁殖力降低。除cat2外，所有基因型的强光都降低了蚜虫的数量。在两种辐照下，cat2和缺乏谷胱甘肽，植物抗毒素和硫代葡萄糖苷的cat2cad2双重突变体中的蚜虫繁殖力都相似。仅在中等光照下才能观察到蚜虫诱导的编码脱落酸相关的阿拉伯糖苷锌蛋白1转录因子的转录本的增加。相反，仅在强光下，在所有基因型中均观察到了蚜虫诱导的编码茉莉酸酯合成酶ALLENE OXIDE CYCLASE 3的转录本的增加。在两种辐照下，除pp2a-b'ζ1-1以外的所有基因型均观察到了蚜虫诱导的氧化还原酶转录因子1 mRNA的增加。因此，蚜虫的繁殖力受到细胞氧化还原信号传导的调节，该信号至少部分地通过依赖PP2A的线粒体介导到细胞核的信号通路。仅在强光下，在所有基因型中均观察到蚜虫诱导的编码茉莉酸酯合成酶ALLENE OXIDE CYCLASE 3的转录本增加。在两种辐照下，除pp2a-b'ζ1-1以外的所有基因型均观察到蚜虫诱导的氧化还原酶转录因子1 mRNA的增加。因此，蚜虫的繁殖力受到细胞氧化还原信号传导的调节，该信号至少部分地通过依赖PP2A的线粒体介导到细胞核的信号通路。仅在强光下，在所有基因型中均观察到蚜虫诱导的编码茉莉酸酯合成酶ALLENE OXIDE CYCLASE 3的转录本增加。在两种辐照下，除pp2a-b'ζ1-1以外的所有基因型均观察到蚜虫诱导的氧化还原酶转录因子1 mRNA的增加。因此，蚜虫的繁殖力受到细胞氧化还原信号传导的调节，该信号至少部分地通过依赖PP2A的线粒体介导到细胞核的信号通路。