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Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop.
Journal of Extracellular Vesicles ( IF 16.0 ) Pub Date : 2019-11-08 , DOI: 10.1080/20013078.2019.1684862
Ashley E Russell 1 , Alexandra Sneider 2 , Kenneth W Witwer 1, 3 , Paolo Bergese 4 , Suvendra N Bhattacharyya 5 , Alexander Cocks 6 , Emanuele Cocucci 7, 8 , Uta Erdbrügger 9 , Juan M Falcon-Perez 10, 11 , David W Freeman 12 , Thomas M Gallagher 13 , Shuaishuai Hu 14 , Yiyao Huang 1, 15 , Steven M Jay 16 , Shin-Ichi Kano 17 , Gregory Lavieu 18 , Aleksandra Leszczynska 19 , Alicia M Llorente 20 , Quan Lu 21 , Vasiliki Mahairaki 3 , Dillon C Muth 1 , Nicole Noren Hooten 12 , Matias Ostrowski 22 , Ilaria Prada 23 , Susmita Sahoo 24 , Tine Hiorth Schøyen 1, 25 , Lifu Sheng 26 , Deanna Tesch 27 , Guillaume Van Niel 28 , Roosmarijn E Vandenbroucke 29, 30 , Frederik J Verweij 28 , Ana V Villar 31 , Marca Wauben 32 , Ann M Wehman 33 , Hang Yin 34 , David Raul Francisco Carter 35 , Pieter Vader 36
Affiliation  

Paracrine and endocrine roles have increasingly been ascribed to extracellular vesicles (EVs) generated by multicellular organisms. Central to the biogenesis, content, and function of EVs are their delimiting lipid bilayer membranes. To evaluate research progress on membranes and EVs, the International Society for Extracellular Vesicles (ISEV) conducted a workshop in March 2018 in Baltimore, Maryland, USA, bringing together key opinion leaders and hands-on researchers who were selected on the basis of submitted applications. The workshop was accompanied by two scientific surveys and covered four broad topics: EV biogenesis and release; EV uptake and fusion; technologies and strategies used to study EV membranes; and EV transfer and functional assays. In this ISEV position paper, we synthesize the results of the workshop and the related surveys to outline important outstanding questions about EV membranes and describe areas of consensus. The workshop discussions and survey responses reveal that while much progress has been made in the field, there are still several concepts that divide opinion. Good consensus exists in some areas, including particular aspects of EV biogenesis, uptake and downstream signalling. Areas with little to no consensus include EV storage and stability, as well as whether and how EVs fuse with target cells. Further research is needed in these key areas, as a better understanding of membrane biology will contribute substantially towards advancing the field of extracellular vesicles.



中文翻译:

EV 生物发生、稳定性、摄取和货物转移中的生物膜:来自 ISEV 膜和 EV 研讨会的 ISEV 立场文件。

旁分泌和内分泌作用越来越多地归因于多细胞生物产生的细胞外囊泡(EV)。EV 的生物发生、内容和功能的核心是它们的脂质双层膜。为了评估膜和 EV 的研究进展,国际细胞外囊泡学会 (ISEV) 于 2018 年 3 月在美国马里兰州巴尔的摩举办了一次研讨会,汇集了根据提交的申请选出的关键意见领袖和实践研究人员。该研讨会还进行了两项科学调查,涵盖了四个广泛的主题:电动汽车的生物发生和释放;电动汽车的采用和融合;用于研究电动汽车膜的技术和策略;以及 EV 转移和功能测定。在这份 ISEV 立场文件中,我们综合了研讨会的结果和相关调查,概述了有关 EV 膜的重要的突出问题并描述了共识领域。研讨会讨论和调查回复表明,虽然该领域取得了很大进展,但仍然存在一些观点分歧的概念。在某些领域存在良好的共识,包括 EV 生物发生、摄取和下游信号传导的特定方面。几乎没有达成共识的领域包括电动汽车的存储和稳定性,以及电动汽车是否以及如何与目标电池融合。这些关键领域需要进一步的研究,因为更好地了解膜生物学将大大有助于推动细胞外囊泡领域的发展。

更新日期:2019-11-08
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