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Phylogenetic and structural diversity of aromatically dense pili from environmental metagenomes.
Environmental Microbiology Reports ( IF 3.3 ) Pub Date : 2019-11-21 , DOI: 10.1111/1758-2229.12809
Marcus S Bray 1, 2 , Jieying Wu 1 , Cory C Padilla 1 , Frank J Stewart 1 , David A Fowle 3 , Cynthia Henny 4 , Rachel L Simister 5, 6 , Katharine J Thompson 5, 6 , Sean A Crowe 2, 5, 6, 7 , Jennifer B Glass 1, 2
Affiliation  

Electroactive type IV pili, or e‐pili, are used by some microbial species for extracellular electron transfer. Recent studies suggest that e‐pili may be more phylogenetically and structurally diverse than previously assumed. Here, we used updated aromatic density thresholds (≥9.8% aromatic amino acids, ≤22‐aa aromatic gaps and aromatic amino acids at residues 1, 24, 27, 50 and/or 51, and 32 and/or 57) to search for putative e‐pilin genes in metagenomes from diverse ecosystems with active microbial metal cycling. Environmental putative e‐pilins were diverse in length and phylogeny, and included truncated e‐pilins in Geobacter spp., as well as longer putative e‐pilins in Fe(II)‐oxidizing Betaproteobacteria and Zetaproteobacteria.

中文翻译:

来自环境宏基因组的芳香密集菌毛的系统发育和结构多样性。

一些微生物物种使用电活性 IV 型菌毛或 e-菌毛进行细胞外电子转移。最近的研究表明,e-菌毛在系统发育和结构上可能比以前假设的更加多样化。在这里,我们使用更新的芳香族密度阈值(≥9.8% 芳香族氨基酸,≤22-aa 芳香族间隙和残基 1、24、27、50 和/或 51 以及 32 和/或 57 处的芳香族氨基酸)来搜索来自具有活跃微生物金属循环的不同生态系统的宏基因组中推定的 e-pilin 基因。环境推定的 e-pilins 在长度和系统发育上是不同的,包括Geobacter spp. 中截短的 e-pilins,以及 Fe(II) 氧化性 Betaproteobacteria和Zetaproteobacteria 中较长的推定 e- pilins
更新日期:2019-11-21
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