This study aimed to examine the mRNA expression, activity, and immunolocalisation of apoptosis/proliferation regulating factors following in vitro exposure of the stroma, white (WFs), and yellowish (YFs) follicles of the chicken ovary to 4-nitrophenol (PNP) or 3-methyl-4-nitrophenol (PNMC). PNMC increased the mRNA expression of caspase-3, -8, Apaf-1, and cytochrome c in the ovarian stroma. The activity of caspase-3, -8, and -9 decreased in WFs in both nitrophenol-treated groups. PNP reduced the number of caspase-3-positive cells in the stromal connective tissue (CT) and the theca interna and externa layers of WFs. In the stroma, the proliferating index decreased in the wall of primary follicles in both nitrophenol-treated groups, however, in the CT, the effect of PNMC was opposite. In the theca interna of WFs, PNP diminished the proliferating index. These results suggest that nitrophenols might impact the development of chicken ovarian follicles by affecting cell death and proliferation.

中文翻译：

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PNP和PNMC的体外作用对母鸡卵巢基质和层前卵泡的凋亡和增殖的影响。

这项研究旨在检查鸡卵巢的间质，白色（WFs）和淡黄色（YFs）卵泡在体外暴露于4-硝基苯酚（PNP）或细胞凋亡后，凋亡/增殖调节因子的mRNA表达，活性和免疫定位。 3-甲基-4-硝基苯酚（PNMC）。PNMC增加了卵巢基质中caspase-3，-8，Apaf-1和细胞色素c的mRNA表达。在两个硝基苯酚处理组中，WF中的caspase-3，-8和-9活性均降低。PNP减少了间质结缔组织（CT）以及WF的内膜和外膜层中caspase-3阳性细胞的数量。在基质中，两个硝基苯酚处理组的初级卵泡壁中的增殖指数均降低，但是在CT中，PNMC的作用相反。在WF的主题世界中，PNP减少了增殖指数。这些结果表明，硝基酚可能通过影响细胞死亡和增殖来影响鸡卵巢卵泡的发育。