In the first step in this study, the effect of 50 mM NaCl was studied on germination percentage of five different Lepidium draba (L. draba) ecotypes, and Rafsanjan ecotype was selected as experimental material as it had the highest germination percentage. In the second step, some biochemical, physiological, and morphological traits along with content of sulforaphane (SFN) as well as the expression level of Cytochorome P450 79F1 (CYP79F1) were evaluated in 14-day-old L. draba sprouts that grew 9 days in the presence of various concentrations of NaCl including 0, 25, 50, 75, and 100 mM. According to the results of this study, germination percentage of Rafsanjan ecotype along with lengths of stem and root were declined with increasing concentrations of NaCl. Ascorbate peroxidase, guaiacol peroxidase, and superoxide dismutase enzymes activity increased up to 75 mM NaCl and then decreased. With increasing the doses of NaCl, concentrations of Na+ and Cl− increased, whereas P, Ca2+, and K+ decreased. Also, accumulation of some oxidative stress parameters including electrolyte leakage, malondialdehyde, other aldehydes, and hydrogen peroxide increased with increasing NaCl concentrations in all samples. Furthermore, contents of total phenolic, total flavonoid, total anthocyanin, total free amino acids, and total soluble carbohydrate were induced with the induction of NaCl concentrations. In this study, SFN formation increased with increasing concentration of sodium chloride up to 75 mM and decreased at higher concentration. In the last step, a partial CYP79F1 mRNA and its protein sequence were identified and registered in GenBank and then changes in the CYP79F1 gene expression levels under 0, 25, 50, 75, and 100 mM NaCl were calculated. The gene expression levels of CYP79F1 also showed the same pattern as was seen for SFN formation under salinity stress.

中文翻译：

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盐胁迫对茼蒿植物酶活性、离子浓度、氧化应激参数、生化性状、萝卜硫素含量及CYP79F1基因表达水平的影响

在本研究的第一步中，研究了 50 mM NaCl 对五种不同的 Lepidium draba (L. draba) 生态型萌发率的影响，并选择 Rafsanjan 生态型作为实验材料，因为它具有最高的萌发率。在第二步中，在生长 9 天的 14 天龄的龙舌兰芽中评估了一些生化、生理和形态特征以及萝卜硫素 (SFN) 的含量以及细胞色素 P450 79F1 (CYP79F1) 的表达水平在各种浓度的 NaCl 存在下，包括 0、25、50、75 和 100 mM。根据这项研究的结果，随着 NaCl 浓度的增加，Rafsanjan 生态型的发芽率以及茎和根的长度下降。抗坏血酸过氧化物酶、愈创木酚过氧化物酶、和超氧化物歧化酶活性增加到 75 mM NaCl 然后降低。随着NaCl剂量的增加，Na+和Cl-的浓度增加，而P、Ca2+和K+的浓度降低。此外，一些氧化应激参数的积累，包括电解质泄漏、丙二醛、其他醛类和过氧化氢，随着所有样品中 NaCl 浓度的增加而增加。此外，总酚、总黄酮、总花青素、总游离氨基酸和总可溶性碳水化合物的含量在 NaCl 浓度的诱导下被诱导。在这项研究中，SFN 的形成随着氯化钠浓度的增加而增加，最高可达 75 mM，而在较高浓度下则减少。在最后一步，鉴定了部分CYP79F1 mRNA及其蛋白质序列并在GenBank中注册，然后计算了0、25、50、75和100 mM NaCl下CYP79F1基因表达水平的变化。CYP79F1 的基因表达水平也显示出与盐胁迫下 SFN 形成相同的模式。