Two analytical methods; high performance liquid chromatography and gas chromatography were used to determine the content of 2-methylquinoxaline, a methylglyoxal-derived agent in sera from cattle with fascioliasis. Methylglyoxal is a highly mutagenic and cytotoxic reactive dicarbonyl compound formed by non-enzymatic fragmentation of triose phosphate GAP and DHAP during glycolysis which regularly contributes to repositioning the energetic balance between physiological and pathological situations. The aim of this study was to propose the MGO as a new biomarker in the bovine fasciolosis. Strongly infected animals showed a correlation between the relatively high levels of Fasciola hepatica anti-f2 antibody and methylglyoxal compared to unharmed animals. Also, an acute hyperglycemia was recorded and closely related to hepatic parenchyma hyperplasia, inflammation, bile ducts obstruction and scléro-fibrous foci formation.Unlike HPLC, which has shown analytical flaws and irregularities, GC-MS remains an excellent diagnostic tool for detecting and quantifying methylglyoxal in biological fluids. The developed method has been validated under FDA guidelines. A full scan-range was set from m/z 39 to 144/999 and the molecular weight of the 2-methylquinoxaline was identified according to NIST Database and ES. Methylglyoxal was the only analyte successfully quantified in a relatively short run time. It was linear over a concentration range of 0.057-5.7  μg.ml-1with mean recoveries and RSD of 118% and 3.63% respectively. The intra and inter-day assays were satisfying and not exceed 3.00%. Results reflect the degree of precision of our method and indicate that MGO was an important contributor to understand the hepatic failure independently of other serum markers.

中文翻译：

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用于血清监测炎性糖毒素（甲基乙二醛）的分析方法GC-MS与LC-UV的开发：牛肝胆管分离病的一种新的生物标志物。

两种分析方法；用高效液相色谱和气相色谱法测定牛筋膜炎的血清中2-甲基喹喔啉（一种甲基乙二醛衍生剂）的含量。甲基乙二醛是一种高度诱变性和细胞毒性的反应性二羰基化合物，由糖酵解过程中磷酸三糖磷酸GAP和DHAP的非酶促裂解形成，通常有助于重新定位生理和病理情况之间的能量平衡。这项研究的目的是提出MGO作为牛筋膜炎的一种新的生物标志物。与未受伤害的动物相比，受到强烈感染的动物表现出相对较高的Fasciola hepatica抗f2抗体水平与甲基乙二醛之间的相关性。此外，还记录了急性高血糖症，它与肝实质增生密切相关，炎症，胆管阻塞和巩膜纤维性病灶形成。与HPLC显示出分析缺陷和不规则性的HPLC不同，GC-MS仍然是检测和定量生物液中甲基乙二醛的出色诊断工具。所开发的方法已根据FDA指南进行了验证。整个扫描范围从m / z 39设置为144/999，并根据NIST数据库和ES鉴定了2-甲基喹喔啉的分子量。甲基乙二醛是在相对较短的运行时间内成功定量的唯一分析物。在0.057-5.7μg.ml-1的浓度范围内呈线性，平均回收率和RSD分别为118％和3.63％。日内和日间分析令人满意，且不超过3.00％。