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Carbo-loading in Coccidioides spp.: A quantitative analysis of CAZyme abundance and resulting glycan populations.
Glycobiology ( IF 4.3 ) Pub Date : 2020-02-19 , DOI: 10.1093/glycob/cwz092
Natalie M Mitchell 1 , Thomas E Grys 1, 2 , Douglas F Lake 1
Affiliation  

Coccidioides spp. are important pneumonia-causing pathogens of the American southwest, but little is known about their glycobiology and how their glycosylations differ from other pneumonia-causing fungi. There is mounting preliminary evidence to suggest genus or even species-specific glycosylations in the fungal kingdom due to the presence of unique carbohydrate-active enzymes (CAZymes) in fungal genomes (Deshpande et al. 2008, Glycobiology, 18(8), 626-637; Karkowska-Kuleta and Kozik 2015, Acta Biochim Pol., 62(3), 339-351). If Coccidioides spp.-specific glycans can be identified, it may be possible to exploit these differences to develop more specific diagnostic approaches and more effective therapeutics. Herein, we i) mined Coccidioides spp. and other pathogenic fungal genomes to identify CAZymes specific for Coccidioides spp., ii) proteomically determined the Coccidioides spp. "CAZome" produced in vivo and in vitro, and iii) utilized glycomics to differentiate Coccidioides genus-specific N-glycans from other pathogenic fungi. As far as we are aware, this is the first proteomic and glycomic comparison of the N-glycomes and CAZomes of different fungal genera during infection in human hosts.

中文翻译:

球孢子菌中的碳载量:对CAZyme丰度和产生的聚糖种群的定量分析。

球虫科 乙型肝炎是美国西南部重要的引起肺炎的病原体,但对其糖生物学以及其糖基化与其他引起肺炎的真菌的区别知之甚少。越来越多的初步证据表明,由于真菌基因组中存在独特的碳水化合物活性酶(CAZymes),因此真菌王国中属甚至物种特异性糖基化(Deshpande et al。2008,Glycobiology,18(8),626- 637; Karkowska-Kuleta和Kozik 2015,Acta Biochim Pol。,62(3),339-351)。如果可以鉴定出球孢菌属特有的聚糖,则有可能利用这些差异来开发更具体的诊断方法和更有效的治疗方法。在此,我们i)开采了球虫科植物。和其他致病性真菌基因组,以鉴定对球虫属特有的Cazymes,ii)蛋白质组学确定了球虫属。在体内和体外产生的“ CAZome”,以及iii)利用糖组学将球虫属的特异性N-聚糖与其他病原性真菌区分开。据我们所知,这是人类宿主感染期间不同真菌属的N-糖基和CAZomes的首次蛋白质组学和糖组学比较。
更新日期:2020-04-17
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