Fusarium head blight (FHB) is a wheat disease with a worldwide prevalence, caused by Fusarium graminearum. Peroxisomes are ubiquitous in eukaryotic cells and are involved in various biochemical phenomena. FgPEX2 and FgPEX12 encode RING-finger peroxins PEX2 and PEX12 in F. graminearum. This study aimed to functionally characterize FgPEX2 and FgPEX12 in F. graminearum. We constructed deletion mutants of FgPEX2 and FgPEX12 via homologous recombination. The ΔPEX2 and ΔPEX12 mutants displayed defects in sexual and asexual development, virulence, cell wall integrity (CWI), and lipid metabolism. Deletion of FgPEX2 and FgPEX12 significantly decreased deoxynivalenol production. Furthermore, fluorescence microscopic analysis of the subcellular localization of GFP-PMP70 and GFP-HEX1 revealed that FgPEX2 and FgPEX12 maintain Woronin bodies. These results show that FgPEX2 and FgPEX12 are required for growth, conidiation, virulence, cell wall integrity, and lipid metabolism in F. graminearum and do not influence their peroxisomes.

中文翻译：

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FgPEX2和FgPEX12在禾谷镰刀菌的毒力和脂质代谢中的作用。

镰刀菌病是由禾谷镰刀菌引起的一种全球流行的小麦病。过氧化物酶体在真核细胞中无处不在，并参与各种生化现象。FgPEX2和FgPEX12编码禾谷镰刀菌中的RING-手指过氧化物酶PEX2和PEX12。这项研究旨在功能上表征禾谷镰刀菌中的FgPEX2和FgPEX12。我们通过同源重组构建了FgPEX2和FgPEX12的缺失突变体。ΔPEX2和ΔPEX12突变体在性和无性发育，毒力，细胞壁完整性（CWI）和脂质代谢方面显示出缺陷。FgPEX2和FgPEX12的删除显着降低了脱氧雪腐烯醇的产生。此外，GFP-PMP70和GFP-HEX1的亚细胞定位的荧光显微镜分析表明，FgPEX2和FgPEX12保持了Woronin体。