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Design Characteristics to Eliminate the Need for Parameter Optimization in Nanoflow ESI-MS.
Journal of the American Society for Mass Spectrometry ( IF 3.2 ) Pub Date : 2019-08-15 , DOI: 10.1007/s13361-019-02301-8
Yang Kang 1 , Bradley B Schneider 1 , Leigh Bedford 1 , Thomas R Covey 1
Affiliation  

The sampling efficiency in electrospray ionization-mass spectrometry (ESI-MS) can be improved by decreasing the liquid flow rate to the nanoflow regime, where it is possible to draw a large fraction of the ESI plume into the mass spectrometer. This mode of operation is typically more difficult than ESI-MS at higher flow rates because it requires careful optimization of a number of parameters to achieve optimal sampling efficiency. In this work, we screened the relative impact on signal intensity and spray stability of factors that included sprayer position, spray electrode protrusion, sprayer tip shape, spray angle relative to the MS inlet, nebulizer gas flow rate, ESI potential, and means for generating the electric field to initiate electrospray. Based on the screening results, we explore the possibility of providing fixed optimal values for many of the key source parameters to eliminate much of the tuning that is required for conventional nanoflow sources. This approach has potential to greatly simplify nanoflow ESI-MS, while providing optimized sensitivity, stability, and robustness, with decreased variability between analyses.

中文翻译:

消除了Nanoflow ESI-MS中参数优化需求的设计特性。

电喷雾电离质谱法(ESI-MS)的采样效率可以通过降低液体流向纳流范围的速度来提高,在这种情况下,可以将大部分ESI羽流吸入质谱仪中。在较高流速下,此操作模式通常比ESI-MS困难,因为它需要仔细优化多个参数才能获得最佳采样效率。在这项工作中,我们筛选了对信号强度和喷雾稳定性的相对影响,这些因素包括喷雾器位置,喷雾电极突出,喷雾器尖端形状,相对于MS入口的喷雾角度,喷雾器气体流速,ESI电位以及产生方法电场引发电喷雾。根据筛选结果,我们探索了为许多关键源参数提供固定最佳值的可能性,以消除传统纳流源所需的许多调整。这种方法具有极大地简化纳流ESI-MS的潜力,同时提供了优化的灵敏度,稳定性和鲁棒性,同时减少了分析之间的差异。
更新日期:2019-08-15
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