RATIONALE Solid-phase microextraction coupled with thermal desorption electrospray ionization tandem mass spectrometry (SPME-TD-ESI-MS/MS) is proposed as a novel method for the rapid quantification of acetaminophen in plasma samples from a pharmacokinetics (PK) study. METHODS Traces of acetaminophen were concentrated on commercial fused-silica fibers coated with a polar polyacrylate (PA) polymer using direct immersion SPME. No agitation, heating, addition of salt, or adjustment of the pH of the sample solution was applied during the extraction. Any acetaminophen absorbed on the SPME fibers was subsequently desorbed and detected by TD-ESI-MS/MS. RESULTS Parameters of the absorption, sensitivity, reproducibility, and linearity for the SPME-TD-ESI-MS/MS method were evaluated. The time required to complete a TD-ESI-MS/MS analysis was less than 30 seconds. Matrix-matching calibration was performed to calculate the concentration of acetaminophen in the sample. A linear calibration curve with a concentration range of 100-10,000 ng/mL was constructed to calculate the quantity of acetaminophen. The SPME-TD-ESI-MS quantification results for acetaminophen in plasma were in good agreement with those obtained by the conventional LC/MS/MS method. CONCLUSIONS With the proposed method, a 10-min SPME time was enough to achieve the lower limit of quantitation (i.e. 100 ng/mL) and for a complete PK profiling of acetaminophen. A shorter extraction time could be achieved by applying agitation, heating, adding salt, or adjusting the pH of the sample solution to enhance analyte absorption efficiency. The time required to detect acetaminophen on the SPME fiber was less than 30 s, allowing the rapid quantification of acetaminophen in plasma with good accuracy.

中文翻译：

---

固相微萃取与热脱附电喷雾电离质谱联用对血浆中对乙酰氨基酚进行快速定量。

RATIONALE固相微萃取与热脱附电喷雾电离串联质谱（SPME-TD-ESI-MS / MS）结合药理动力学（PK）研究，是一种快速定量测定血浆样品中对乙酰氨基酚的新方法。方法使用直接浸入式SPME，将痕量对乙酰氨基酚浓缩在涂有极性聚丙烯酸酯（PA）聚合物的商用熔融石英纤维上。在提取过程中，不进行搅拌，加热，添加盐或调节样品溶液的pH值。随后将吸附在SPME纤维上的任何对乙酰氨基酚解吸并通过TD-ESI-MS / MS检测。结果评估了SPME-TD-ESI-MS / MS方法的吸收，灵敏度，重现性和线性参数。完成TD-ESI-MS / MS分析所需的时间少于30秒。进行基质匹配校准以计算样品中对乙酰氨基酚的浓度。构建浓度范围为100-10,000 ng / mL的线性校准曲线以计算对乙酰氨基酚的量。血浆中对乙酰氨基酚的SPME-TD-ESI-MS定量结果与常规LC / MS / MS方法获得的结果一致。结论使用所提出的方法，10分钟的SPME时间足以达到定量下限（即100 ng / mL），并完成对乙酰氨基酚的PK分析。可以通过搅拌，加热，添加盐或调节样品溶液的pH值来提高分析物的吸收效率，从而缩短提取时间。