During recombinant production of asymmetric IgG-like bispecific antibodies (bsAbs), various by-products are often observed due to unbalanced chain expression and incorrect chain pairing. Among them, half antibody and homodimer are found with high frequency. In this work, with a case study we demonstrated that Capto MMC ImpRes mixed-mode chromatography can effectively remove these two by-products as well as antibody aggregates under optimized conditions. This makes MMC ImpRes a powerful tool for bsAb purification.

中文翻译：

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使用MMC ImpRes混合模式色谱在双特异性抗体纯化过程中去除半抗体，孔-同型二聚体和聚集体。

在重组生产不对称IgG样双特异性抗体（bsAbs）期间，由于链表达不平衡和链配对不正确，经常会观察到各种副产物。其中，发现半抗体和同型二聚体的频率很高。在这项工作中，通过案例研究，我们证明了Capto MMC ImpRes混合模式色谱法可以在优化的条件下有效去除这两种副产物以及抗体聚集体。这使MMC ImpRes成为纯化bsAb的强大工具。