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Septins are required for reproductive propagule development and virulence of the maize pathogen Cochliobolus heterostrophus.
Fungal Genetics and Biology ( IF 3 ) Pub Date : 2019-11-04 , DOI: 10.1016/j.fgb.2019.103291
Xianghui Zhang 1 , Jonathan B González 2 , B Gillian Turgeon 2
Affiliation  

Septins are highly conserved GTP-binding proteins that function in cell cytokinesis, polarity and morphogenesis. To evaluate the roles of these proteins in inoculum health and disease, mutants deleted for each of five septin proteins (Cdc3, Cdc10, Cdc11, Cdc12, and Cdc100) were characterized in the ascomycete Cochliobolus heterostrophus for ability to develop asexual and sexual spores and for virulence to the host maize. Strains deleted for CDC3, CDC10, CDC11, and CDC12 genes showed significant changes in hyphal growth, and in development of conidia and ascospores compared to the wild-type strain. Conidia had dramatically reduced numbers of septa and rates of germination, while ascospore development was blocked in the meiotic process. Although asci were produced, wild-type ascospores were not. When equal numbers of conidia from wild type and mutants were used to inoculate maize, cdc10 mutants showed reduced virulence compared to the wild-type strain and other mutants. This reduced virulence was demonstrated to be correlated with lower germination rate of cdc10 mutant conidia. When adjusted for germination rate, virulence was equivalent to the wild-type strain. Double mutants (cdc3cdc10, cdc3cdc11) showed augmented reduced growth phenotypes. cdc100 mutants were wild type in all assays. Taken together, these findings indicate that all four conserved septin proteins play a major role in reproductive propagule formation and that mutants with deletions of CDC10 are reduced in virulence to the host maize.

中文翻译:

分离素是玉米繁殖病原体Cochliobolus heterostrophus繁殖繁殖和毒力所必需的。

Septins是高度保守的GTP结合蛋白,在细胞胞质分裂,极性和形态发生中起作用。为了评估这些蛋白质在接种物健康和疾病中的作用,在子囊菌Cochliobolus heterostrophus中鉴定了5种Septin蛋白(Cdc3,Cdc10,Cdc11,Cdc12和Cdc100)中每种缺失的突变体的无性和有性孢子发育能力以及对寄主玉米的毒力。与野生型菌株相比,针对CDC3,CDC10,CDC11和CDC12基因缺失的菌株在菌丝生长以及分生孢子和子囊孢子的发育中显示出显着变化。分生孢子显着减少了隔数和发芽率,而子囊孢子的发育在减数分裂过程中受到了阻碍。尽管产生了asci,但没有产生野生型子囊孢子。当使用来自野生型和突变体的等分生孢子来接种玉米时,与野生型菌株和其他突变体相比,cdc10突变体显示出降低的毒力。已证明这种降低的毒力与cdc10突变体分生孢子的较低发芽率相关。调整发芽率后,毒力相当于野生型菌株。双突变体(cdc3cdc10,cdc3cdc11)显示出增加的减少的生长表型。在所有测定中,cdc100突变体均为野生型。综上所述,这些发现表明,所有四个保守的septin蛋白在繁殖繁殖体的形成中都起着重要作用,并且带有CDC10缺失的突变体对宿主玉米的毒力降低。已证明这种降低的毒力与cdc10突变体分生孢子的较低发芽率相关。调整发芽率后,毒力相当于野生型菌株。双突变体(cdc3cdc10,cdc3cdc11)显示出增加的减少的生长表型。在所有测定中,cdc100突变体均为野生型。综上所述,这些发现表明,所有四个保守的septin蛋白在繁殖繁殖体的形成中都起着重要作用,并且带有CDC10缺失的突变体对宿主玉米的毒力降低。事实证明,这种降低的毒力与cdc10突变体分生孢子的较低发芽率相关。调整发芽率后,毒力相当于野生型菌株。双突变体(cdc3cdc10,cdc3cdc11)显示出增加的减少的生长表型。在所有测定中,cdc100突变体均为野生型。综上所述,这些发现表明,所有四个保守的septin蛋白在繁殖繁殖体的形成中都起着重要作用,并且带有CDC10缺失的突变体对宿主玉米的毒力降低。
更新日期:2019-11-04
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