Newcastle disease virus (NDV) causes huge economic loss to the poultry industry due to high mortality and morbidity. The present study aimed to assess the protective role of novel phosphorylated analogue ABC-1 in vivo in NDV-infected chickens through the inhibition of fusion protein. Both NDV-induced oxidative damage and protective role of novel phosphorylated ABC-1 were evaluated in vital organs such as the liver and lung of chickens. Enzyme linked immunosorbent assay (ELISA) results showed that protein oxidation and nitration levels were significantly raised in NDV-infected tissues compared to healthy controls, whereas these levels were reduced significantly (P < 0.05) in birds treated with phosphorylated compounds compared to the NDV-infected group alone. Additional investigation with double immunofluorescence showed that the large amount of immuno colocalization and Western blot analysis also confirmed this observation through its band pattern in NDV-infected birds compared to healthy birds, whereas these alterations were reduced in treatment with novel phosphorylated ABC-1. The expression of fusion glycoprotein was studied by immuno colocalization, PCR, and flow cytometry, and results demonstrated that the novel phosphorylated analogues reduced the expression of fusion glycoprotein. These results put forth that novel phosphorylated ABC-1 protects chickens from NDV-induced pathogenesis, protein oxidation/nitration, and exerts potent antiviral activity.

中文翻译：

---

磷酸化的阿巴卡韦类似物（ABC-1）对鸡新城疫病毒引起的发病机理具有改善作用。

由于高死亡率和高发病率，新城疫病毒（NDV）对家禽业造成巨大的经济损失。本研究旨在通过抑制融合蛋白来评估新型磷酸化类似物ABC-1在NDV感染鸡体内的保护作用。在鸡的肝脏和肺等重要器官中，都评估了NDV诱导的氧化损伤和新型磷酸化ABC-1的保护作用。酶联免疫吸附试验（ELISA）结果表明，与健康对照组相比，NDV感染组织中蛋白质的氧化和硝化水平显着提高，而与NDV-仅感染组。双重免疫荧光的其他研究表明，与健康鸟类相比，NDV感染鸟类的大量免疫共定位和蛋白质印迹分析也通过其带谱模式证实了这一观察结果，而在新型磷酸化ABC-1处理中，这些改变得以减少。通过免疫共定位，PCR和流式细胞术研究了融合糖蛋白的表达，结果表明新型的磷酸化类似物降低了融合糖蛋白的表达。这些结果表明，新型磷酸化的ABC-1保护鸡免受NDV诱导的发病机制，蛋白质氧化/硝化，并发挥有效的抗病毒活性。