Algal lipids have shown promising feedstock to produce biodiesel due to higher energy content, higher cetane number, and renewable nature. However, at present, the lipid productivity is too low to meet the commercial needs. Various approaches can be employed to enhance the lipid content and lipid productivity in microalgae. Stress manipulation is an attractive option to modify the algal lipid content, but it faces the drawback of time-consuming production processing and lack of information about molecular mechanisms related to triacylglycerides production in response to stress. Developing the robust hyper lipid accumulating algal strains has gained momentum due to advances in metabolic engineering and synthetic biology tools. Understanding the molecular basis of lipid biosynthesis followed by reorienting the related pathways through genomic modification is an alluring strategy that is believed to achieve the industrial and economic robustness. This review portrays the use of integrated OMIC approaches to elucidate the molecular mechanisms of strain adaptability in response to stress conditions, and identification of molecular pathways that should become novel targets to develop novel algal strains. Moreover, an update on the metabolic engineering approaches to improve the lipid production in microalgae is also provided.

中文翻译：

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工程化脂质生物合成的代谢途径，以开发出健壮的微藻菌株来生产生物柴油。

由于较高的能量含量，较高的十六烷值和可再生的性质，藻类脂质已显示出有希望的原料来生产生物柴油。然而，目前，脂质生产率太低而不能满足商业需求。可以采用各种方法来提高微藻中的脂质含量和脂质生产率。应力操纵是改变藻类脂质含量的一种有吸引力的选择，但它面临耗时的生产过程以及缺乏有关与响应压力而生产甘油三酯相关的分子机制的信息的缺点。由于代谢工程和合成生物学工具的发展，开发强大的高脂质积累藻类菌株已获得发展势头。了解脂质生物合成的分子基础，然后通过基因组修饰来重新定向相关途径，这是一种诱人的策略，据信可以实现工业和经济上的稳健性。这篇综述描绘了使用集成的OMIC方法阐明应变适应性的分子机制，以应对压力条件，并鉴定了应该成为开发新型藻类菌株的新靶标的分子途径。此外，还提供了有关代谢工程方法的更新，以改善微藻中的脂质产生。和鉴定应该成为开发新型藻类菌株的新靶标的分子途径。此外，还提供了有关代谢工程方法的更新，以改善微藻中的脂质产生。和鉴定应该成为开发新型藻类菌株的新靶标的分子途径。此外，还提供了有关代谢工程方法的更新，以改善微藻中的脂质产生。