Primary cell cultures are challenging, but reliable model reflecting tumor response in vitro. The study was designed to examine if the increased electropermeabilization can overcame initial drug insensitivity in chondrosarcoma cells from lung metastasis. We established a primary cell culture and evaluated the cytotoxic impact of four drugs-cisplatin (CDDP), camptothecin, 2-methoxyestradiol, and leucovorin calcium (LeuCa). After determination of parameters allowing for electropermeabilization, we performed electrochemotherapy in vitro with the least toxic drugs-CDDP and LeuCa. Although combining CDDP and leucovorin together increased their toxicity and supported apoptosis, application of pulsed electric fields (PEFs) brought no advantage for their efficacy. The study emphasizes the need for introduction of primary cell cultures into studies on pulse electric fields as model frequently less sensitive to PEF-based treatments than continuous cell lines.

中文翻译：

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来自原代细胞培养的转移性软骨肉瘤细胞的电透化。

原代细胞培养具有挑战性，但可靠的模型反映了体外的肿瘤反应。该研究旨在检查增加的电通透性​​是否可以克服由于肺转移引起的软骨肉瘤细胞中最初的药物不敏感性。我们建立了原代细胞培养并评估了四种药物顺铂（CDDP），喜树碱，2-甲氧基雌二醇和亚叶酸钙（LeuCa）的细胞毒性作用。确定允许电透化的参数后，我们用毒性最低的药物CDDP和LeuCa进行了体外电化学疗法。尽管将CDDP和亚叶酸钙结合在一起可增加其毒性并支持细胞凋亡，但脉冲电场（PEF）的应用对其功效没有任何好处。