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Diagnosing ovarian cancer by identifying SCC-antigen on a multiwalled carbon nanotube-modified dielectrode sensor.
Biotechnology and Applied Biochemistry ( IF 2.8 ) Pub Date : 2019-09-12 , DOI: 10.1002/bab.1808
Ting Liang 1 , Qiuzhi Qu 2 , Yuhua Chang 3 , Subash Chandra Bose Gopinath 4, 5 , Xian Tong Liu 3
Affiliation  

Ovarian cancer starts in the ovaries in its earlier stages and then spreads to the pelvis, uterus, and abdominal region. The success of an ovarian cancer treatment depends on the stage of the cancer and the diagnostic system. Squamous cell carcinoma antigen (SCC-Ag) is one of the most efficient cancer biomarkers, and elevated levels of SCC-Ag in ovarian cancer cells have been used to identify ovarian cancer. Carbon is a potential material for biosensing applications due to its thermal, electrical, and physical properties. Multiwalled carbon nanotubes (MWCNTs) are carbon-based materials that can be used here to detect SCC-Ag. Anti-SCC-Ag antibody was immobilized on the amine-modified MWCNT dielectric sensing surface to detect SCC-Ag. The uniformity of the surface structure was measured with a 3D nanoprofiler, and the results confirmed the detection of SCC-Ag at ∼80 pM. The specific detection of SCC-Ag was confirmed with two control proteins (factor IX and human serum albumin), and the system did not show biofouling. This experimental set-up with MWCNTs a dielectric sensing surface can lead to the detection of ovarian cancer in its initial stages.

中文翻译:

通过在多壁碳纳米管修饰的双电极传感器上鉴定SCC抗原来诊断卵巢癌。

卵巢癌开始于卵巢的早期阶段,然后扩散到骨盆,子宫和腹部区域。卵巢癌治疗的成功取决于癌症的阶段和诊断系统。鳞状细胞癌抗原(SCC-Ag)是最有效的癌症生物标志物之一,卵巢癌细胞中SCC-Ag水平升高已被用于鉴定卵巢癌。碳由于其热,电和物理特性而成为生物传感应用的潜在材料。多壁碳纳米管(MWCNT)是基于碳的材料,可在此处用于检测SCC-Ag。将抗SCC-Ag抗体固定在胺修饰的MWCNT介电传感表面上,以检测SCC-Ag。用3D纳米轮廓仪测量表面结构的均匀性,结果证实在〜80 pM时检测到SCC-Ag。用两种对照蛋白(因子IX和人血清白蛋白)确认了SCC-Ag的特异性检测,并且该系统未显示生物结垢。使用MWCNTs进行电介质传感表面的这种实验装置可以在其初始阶段检测出卵巢癌。
更新日期:2020-01-09
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