Raspberry ketone is a primary aroma component of the red raspberry. The glycosylation of this compound is a potential approach used to improve its pharmaceutical properties. In this work, raspberry ketone glycosides are produced in bacteria for the first time. Bacillus licheniformis PI15, an organic solvent‐tolerant glycosyltransferase‐producing strain, was isolated from chemically polluted soil. The cloning and heterologous expression of a glycosyltransferase, which was designated PI‐GT1, in Escherichia coli BL21 resulted in the expression of an active and soluble protein that accounted for 15% of the total cell protein content. Purified PI‐GT1 was highly active and stable over a broad pH range (6.0–10.0) and showed excellent pH stability. PI‐GT1 maintained almost 60% of its maximal activity after 3 H of incubation at 20–40 °C and demonstrated optimal activity at 30 °C. Additionally, PI‐GT1 displayed high stability and activity in the presence of hydrophilic solvents with log P  ≤ −0.2 and retained more than 80% of its activity after 3 H of treatment. Supplementation with 10% DMSO markedly improved the glycosylation of raspberry ketone, resulting in a value 26 times higher than that in aqueous solution. The organic solvent‐tolerant PI‐GT1 may have potential uses in industrial chemical and pharmaceutical synthesis applications.

中文翻译：

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地衣芽孢杆菌PI15的有机溶剂耐受性糖基转移酶的生化特征及其在树莓酮糖苷生产中的潜在应用。

覆盆子酮是红覆盆子的主要香气成分。该化合物的糖基化是用于改善其药物性质的潜在方法。在这项工作中，树莓酮糖苷首次在细菌中产生。从化学污染的土壤中分离到地衣芽孢杆菌PI15，一种耐有机溶剂的糖基转移酶生产菌株。糖基转移酶在大肠杆菌中的克隆和异源表达BL21导致表达一种活性和可溶性蛋白，该蛋白占总细胞蛋白含量的15％。纯化的PI‐GT1在广泛的pH范围（6.0–10.0）中具有很高的活性和稳定性，并具有出色的pH稳定性。在20–40°C下孵育3 H后，PI‐GT1保持了其最大活性的近60％，并在30°C下表现出最佳活性。此外，在具有log P的亲水性溶剂存在下，PI‐GT1表现出高稳定性和活性 ≤-0.2，并在3 H处理后保留了80％以上的活性。补充10％DMSO可以显着改善覆盆子酮的糖基化作用，其值比水溶液高26倍。耐有机溶剂的PI‐GT1在工业化学和药物合成应用中可能具有潜在用途。