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Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide.
Biotechnology and Applied Biochemistry ( IF 2.8 ) Pub Date : 2019-09-09 , DOI: 10.1002/bab.1817
Zhangjun Chen 1, 2 , Zhenbin Chen 1, 2 , Chunli Liu 1, 2 , Xudong Wang 1, 2 , Yongshan Zhou 1, 2 , Runtian Wang 1, 2
Affiliation  

In this work, TiO2 , which was modified by glutaraldehyde, was adopted as the carrier; the penicillin G acylase (PGA) was immobilized and the influence of immobilized conditions, such as pH of solution, the concentration of PGA, the immobilization temperature, and the reaction time, on the catalytic performance of the immobilized PGA was investigated and optimized. During this process, potassium penicillin G (PG) was chosen as substrate, and the quantity of 6-aminopenicillanic acid (6-APA) produced by PG at the temperature of 25 °C for 3 Min in neutral solution was conscripted as the evaluation foundation, indexes, containing the loading capacity (ELC), the activity (EA), and activity retention rate (EAR), were calculated based on quantities of produced 6-APA and compared with finding out the suitable conditions. Results showed that when the solution pH, PGA concentration, immobilization temperature, and reaction time were 8.0, 2.5% (v/v), 35 °C, and 24 H, respectively, ELC, EA, and EAR presented optimal values of 9,190 U, 14,969 U/g, and 88.5% relatedly. After that, the stability and reusability of immobilized PGA were studied, and the results documented that the pH resistance, thermal stability, and storage stability of immobilized PGA were significantly improved. This work provided technique support for the practical application of immobilized PGA carrier.

中文翻译:

固定在戊二醛修饰的二氧化钛上的青霉素G酰基转移酶的优化。

在这项工作中,采用戊二醛改性的TiO2作为载体。固定化了青霉素G酰基转移酶(PGA),研究并优化了固定化条件如溶液的pH值,PGA的浓度,固定化温度和反应时间对固定化PGA的催化性能的影响。在此过程中,以青霉素钾(PG)为底物,以PG在中性溶液中于25℃温度下3分钟生成的6-氨基青霉酸(6-APA)的量为评价依据。 ,基于产生的6-APA的数量计算包含负荷能力(ELC),活性(EA)和活性保留率(EAR)的指数,并与找出合适的条件进行比较。结果表明,当溶液的pH,PGA浓度,固定化温度和反应时间分别为8.0、2.5%(v / v),35°C和24 H时,ELC,EA和EAR的最佳值为9,190 U分别为14969 U / g和88.5%。此后,研究了固定化PGA的稳定性和可重复使用性,结果表明固定化PGA的耐pH性,热稳定性和储存稳定性得到了显着改善。这项工作为固定化PGA载体的实际应用提供了技术支持。结果表明,固定化PGA的耐pH性,热稳定性和储存稳定性均得到了显着提高。这项工作为固定化PGA载体的实际应用提供了技术支持。结果表明,固定化PGA的耐pH性,热稳定性和储存稳定性均得到了显着提高。这项工作为固定化PGA载体的实际应用提供了技术支持。
更新日期:2020-01-09
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