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Development of sensitive benzofurazan-based spectrometric methods for analysis of spectinomycin in vials and human biological samples.
Luminescence ( IF 2.9 ) Pub Date : 2019-08-05 , DOI: 10.1002/bio.3688
Samiha A Hussein 1 , Baher I Salman 2 , Marwa F B Ali 1 , Mostafa A Marzouq 2
Affiliation  

Spectinomycin hydrochloride (SPEC) is an aminoglycoside antibiotic that has a broad spectrum against several bacterial strains of humans and veterinary infections. However, SPEC lacks a fluorophore or chromophore and this lack makes its analysis a challenge. Our study provides a simple, sensitive and low-cost spectrofluorimetric/spectrophotometric method based on the reaction between secondary amine groups and a benzofurazan reagent using borate buffer (pH 9.2). The yielding compound was measured fluorimetrically at 530 nm (excitation at 460 nm) with colorimetry at 410 nm. The calibration curves ranged from 30 to 400 ng ml-1 and from 0.2 to 6 μg ml-1 for spectrofluorimetric and spectrophotometric analyses, respectively. The limits of detection were calculated to be 4.15 ng ml-1 and 0.05 μg ml-1 for spectrofluorimetric and spectrophotometric processes, respectively. The ultra-affectability and high selectivity of the proposed method permitted analysis of SPEC in the dosage form and in human plasma samples, with good recoveries of about 101.19 and 97.11%, respectively, without any matrix interference. The proposed strategy was validated using International Conference on Harmonization standards and validated bioanalytically using USFDA recommendations.

中文翻译:

灵敏的基于苯并呋喃酮的光谱分析方法的开发,用于分析小瓶和人类生物样品中的壮观霉素。

壮观霉素盐酸盐(SPEC)是一种氨基糖苷类抗生素,对人类的几种细菌菌株和兽医感染具有广谱性。但是,SPEC缺少荧光团或发色团,因此缺乏对其分析的挑战。我们的研究提供了一种简单,灵敏且低成本的荧光分光光度法/分光光度法,该方法基于仲胺基与使用硼酸盐缓冲液(pH 9.2)的苯并呋喃山试剂之间的反应。在530nm(在460nm处激发)下用荧光法用410nm处的比色法对得到的化合物进行荧光测定。对于分光荧光分析和分光光度分析,校准曲线的范围分别为30到400 ng ml-1和0.2到6μgml-1。计算的检出限为4.15 ng ml-1和0。05μgml-1分别用于分光荧光法和分光光度法。所提方法的超亲和力和高选择性使得可以对剂型和人血浆样品中的SPEC进行分析,分别具有约101.19%和97.11%的良好回收率,而不会受到任何基质干扰。拟议的策略已使用国际协调会议标准进行了验证,并使用USFDA的建议进行了生物分析验证。
更新日期:2019-12-11
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