Industrial hempseed is an important agricultural crop with increasing potential for food applications. The objective of this study was to determine the different biological activities of hempseed coproducts after hempseed oil extraction and hempseed protein isolate precipitation. Hempseed oil was extracted by n‐hexane and the defatted meal was dried. The hempseed protein isolate was prepared by alkaline pH solubilization followed by isoelectric precipitation. Two coproducts were obtained by freeze drying the precipitate after alkaline solubilization and freeze drying the supernatant after isoelectric precipitation. The coproducts were called hempseed meal after protein isolation (HM‐PI) and hempseed supernatant after protein isolation (HS‐PI). The biological activities of HM‐PI and HS‐PI were determined by measuring their antioxidant properties, antiproliferative properties against two human colon cancer cell lines, and anti‐inflammatory activities in lipopolysaccharide‐induced RAW 264.7 macrophages. The results showed that HS‐PI has better biological activities than HM‐PI, which correlated positively with the concentrations of soluble proteins, soluble polyphenols, tannins, and flavonoids. Our results showed for the first time that coproducts of hempseed processing can be potential sources of food ingredients with health‐promoting properties.

中文翻译：

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大麻油和大麻籽分离蛋白生产副产物水提物的比较生物活性测定

工业大麻是重要的农作物，具有增加食品应用潜力的潜力。这项研究的目的是确定大麻油提取和大麻蛋白分离物沉淀后，大麻副产物的不同生物活性。大麻油经n提取己烷和脱脂粗粉干燥。大麻蛋白分离物的制备是通过碱pH值增溶，然后进行等电沉淀。碱溶解后将沉淀物冷冻干燥，等电沉淀后将上清液冷冻干燥，从而得到两种副产物。副产物被称为蛋白质分离后的大麻粕（HM-PI）和蛋白质分离后的HS-PI大麻籽上清液。HM-PI和HS-PI的生物学活性通过测量它们的抗氧化特性，对两种人结肠癌细胞系的抗增殖特性以及脂多糖诱导的RAW 264.7巨噬细胞的抗炎活性来确定。结果表明，HS-PI具有比HM-PI更好的生物活性，这与可溶性蛋白的浓度呈正相关，可溶性多酚，单宁和类黄酮。我们的结果首次表明，大麻加工的副产品可以成为具有健康促进特性的食品成分的潜在来源。