The chemical shift difference, Δσ, between the methylene and hydroxyl protons in the high resolution 1H nuclear magnetic resonance spectrum of ethylene glycol is shown to be pressure dependent. The equilibrium Δσ values for ethylene glycol are reported as a function of temperature and pressure between ambient conditions, 323 K and 2 kbar, respectively. This surface is used along with Δσ values measured in response to a rapid pressure increase to calculate a temperature rise that is used to infer a temperature change for water that is consistent with theoretical estimates. This work implies that compression heating and decompression cooling are not significant enough to interfere with pressure induced protein folding studies.

中文翻译：

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乙二醇中压缩加热和减压冷却的实验估计

乙二醇的高分辨率 1H 核磁共振谱中亚甲基和羟基质子之间的化学位移差异 Δσ 显示为与压力有关。乙二醇的平衡 Δσ 值报告为环境条件之间温度和压力的函数，分别为 323 K 和 2 kbar。该表面与响应快速压力增加而测量的 Δσ 值一起使用，以计算温升，该温升用于推断与理论估计值一致的水温变化。这项工作意味着压缩加热和减压冷却不足以干扰压力诱导的蛋白质折叠研究。