Platelet function is regulated by finely tuned phosphoprotein signals. Subtle aberrations in signaling can cause platelet hyperactivity and severe cardiovascular events. Mapping phosphorylation profiles in health and disease could accelerate antiplatelet discovery and enhance cardiovascular management, but traditional assays are ill-suited to clinical application as they are laborious and low throughput. Recent advances in multiplex flow cytometry (barcoding) allow the rapid acquisition of highly batched samples with standard laboratory equipment. However, many assays have not been standardized, and success is largely dependent on protocol/reagent selection. Accordingly, we review the technical steps that are key to success with an emphasis on fixation, permeabilization, staining, controls, and data visualization.

中文翻译：

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血小板中的磷酸流式细胞术和条形码：技术和分析方面的考虑。

血小板功能由微调的磷蛋白信号调节。信号中的细微畸变会导致血小板过度活跃和严重的心血管事件。在健康和疾病中绘制磷酸化谱图可以加速抗血小板发现并增强心血管管理，但是传统的测定方法费力且通量低，因此不适合临床应用。多重流式细胞术（条形码）的最新进展使得可以使用标准实验室设备快速采集大量样品。但是，许多测定方法尚未标准化，成功与否在很大程度上取决于方案/试剂的选择。因此，我们回顾了成功的关键技术步骤，重点是固定，通透性，染色，对照和数据可视化。