BACKGROUND Hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) represent the most common primary liver malignancies whose outcome is influenced by the immune response. METHODS In this study, we have functionally characterized, by flow cytometry, circulating myeloid dendritic cells (mDCs) and FcεRI+ monocytes in a group of healthy individuals (n = 10) and in a group of patients with HCC (n = 19) and CCA (n = 8), at the time point of the surgical resection (T0) and once the patient had recovered from surgery (T1). Moreover, we proceeded to a more in depth phenotypic characterization of the FcεRI+ monocyte subpopulation. RESULTS A significant decrease in the frequency of TNFα producing FcεRI+ monocytes and mDCs in HCC and CCA patients when compared to the group of healthy individuals was observed, and a close association between FcεRI+ monocytes and mDCs dysfunction was identified. In addition, the phenotypic characteristics of FcεRI+ monocytes from healthy individuals strongly suggest that this population drives to mDCs, which matches with the fact that both populations are functionally affected. CONCLUSIONS The frequency and the function of circulating mDCs and FcεRI+ monocytes are affected in both HCC and CCA patients, and FcεRI+ monocytes could represent those fated to become mDCs. © 2019 International Clinical Cytometry Society.

中文翻译：

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肝细胞癌和胆管癌患者中循环FcεRI+单核细胞和髓样树突状细胞的功能受损。

背景技术肝细胞癌（HCC）和胆管癌（CCA）代表最常见的原发性肝恶性肿瘤，其结果受免疫反应影响。方法在本研究中，我们通过流式细胞仪对一组健康个体（n = 10）和一组肝癌（n = 19）和CCA患者的循环髓样树突状细胞（mDCs）和FcεRI+单核细胞进行了功能表征。 （n = 8），在手术切除的时间点（T0），一旦患者从手术中康复（T1）。此外，我们进行了更深入的FcεRI+单核细胞亚群的表型表征。结果与健康个体相比，HCC和CCA患者中产生TNFα的FcεRI+单核细胞和mDC的频率显着降低，并发现FcεRI+单核细胞与mDCs功能障碍密切相关。此外，来自健康个体的FcεRI+单核细胞的表型特征强烈表明，该人群驱使进入mDC，这与两个人群均受到功能影响这一事实相吻合。结论在HCC和CCA患者中，循环mDC和FcεRI+单核细胞的频率和功能均受到影响，而FcεRI+单核细胞可能代表注定成为mDC的那些。©2019国际临床细胞计量学会。结论在HCC和CCA患者中，循环mDC和FcεRI+单核细胞的频率和功能均受到影响，而FcεRI+单核细胞可能代表注定成为mDC的那些。©2019国际临床细胞计量学会。结论在HCC和CCA患者中，循环mDC和FcεRI+单核细胞的频率和功能均受到影响，而FcεRI+单核细胞可能代表注定成为mDC的那些。©2019国际临床细胞计量学会。