BACKGROUND Neurotuberculosis is one of the commonest HIV associated opportunistic infections of the central nervous system in India. HIV-TB coinfection may lead to altered frequencies of T cells, thereby influencing the course and progression of the disease. METHODS We examined the frequencies of T cell subsets in HIV infected individuals with neurotuberculosis (HIV+nTB+) as compared to individuals with HIV associated systemic TB (HIV+sTB+), asymptomatic HIV (HIV+TB-), non-HIV neuro TB (HIV-nTB+), non-HIV systemic TB (HIV-sTB+), and healthy controls (HIV-TB-). Activation and senescence profiles of CD4 and CD8 T cells and memory subsets in peripheral blood mononuclear cells were studied by flow cytometry. RESULTS The significant observations among the T cell subsets in HIV+nTB+ were: (1) Naïve T cells: decreased CD4 T cells compared to HIV-sTB+ (P = 0.005); decreased CD8 T cells compared to HIV-nTB+ and HIV-TB- (P ≤ 0.007), (2) Memory T cells: expanded CD4 TEMRA cells compared to HIV-nTB+, HIV-sTB+, and HIV-TB- (P ≤ 0.003); expanded CD8 TEMRA cells compared to HIV-nTB+ and HIV-TB- (P ≤ 0.005), (3) Activated T cells: higher CD4 T cells compared to HIV-nTB+, HIV-sTB+, and HIV-TB- (P ≤ 0.004); higher CD8 T cells compared to HIV + TB-, HIV-nTB+, HIV-sTB+, and HIV-TB- (P ≤ 0.001), and (4) Senescent T cells: increased CD8 T cells compared to HIV-nTB+ and HIV-TB- groups (P = 0.000). CONCLUSIONS Increased activation compared to HIV+TB-, HIV-nTB+, HIV-sTB+, and HIV-TB- groups and increased senescence compared to HIV-nTB+ and HIV-TB- groups were observed in CD8 T cells in HIV+nTB+, suggesting that the frequencies of these T cell subsets are altered to a greater extent in these individuals. © 2018 International Clinical Cytometry Society.

中文翻译：

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HIV神经结核合并感染中T细胞表型的改变。

背景技术神经结核是印度中枢神经系统最常见的HIV相关机会性感染之一。HIV-TB合并感染可能导致T细胞频率改变，从而影响疾病的进程和进展。方法我们检查了感染了神经结核（HIV + nTB +）的HIV感染者与未感染HIV的系统性结核病（HIV + sTB +），无症状HIV（HIV + TB-），非HIV神经结核病（T细胞）的T细胞亚群的频率HIV-nTB +），非HIV系统性结核（HIV-sTB +）和健康对照（HIV-TB-）。通过流式细胞术研究了外周血单个核细胞中CD4和CD8 T细胞以及记忆亚群的活化和衰老特征。结果HIV + nTB +的T细胞亚群中的重要观察结果是：（1）幼稚T细胞：与HIV-sTB +相比，CD4 T细胞减少（P = 0.005）；与HIV-nTB +和HIV-TB-相比减少了CD8 T细胞（P≤0.007），（2）记忆T细胞：与HIV-nTB +，HIV-sTB +和HIV-TB-相比，CD4 TEMRA细胞扩增了（P≤0.003 ）; 与HIV-nTB +和HIV-TB-相比扩增的CD8 TEMRA细胞（P≤0.005），（3）活化T细胞：与HIV-nTB +，HIV-sTB +和HIV-TB-相比，CD4 T细胞更高（P≤0.004 ）; CD8 T细胞比HIV + TB-，HIV-nTB +，HIV-sTB +和HIV-TB-高（P≤0.001），以及（4）衰老T细胞：与HIV-nTB +和HIV-相比，CD8 T细胞增加TB组（P = 0.000）。结论在HIV + nTB +的CD8 T细胞中，与HIV + TB-，HIV-nTB +，HIV-sTB +和HIV-TB-组相比，活化增强，与HIV-nTB +和HIV-TB-组相比，衰老增加，提示这些T细胞亚群的频率在这些个体中发生了较大程度的改变。©2018国际临床细胞计量学会。