Regulatory assessment of the bioaccumulation from water is commonly based on bioconcentration factors (BCFs) derived from fish flow-through tests. Such experiments require many laboratory animals and are time-consuming and costly. An alternative test setup for organic, neutral compounds using the amphipod Hyalella azteca was recently suggested, resulting in BCF values which show a strong correlation with fish BCF data. In the present study, the bioconcentration potential of the ionic compound laurate was elucidated in H. azteca. The sodium salt of 1-14 C laurate was applied to H. azteca in a flow-through and a semistatic approach. Because of rapid biodegradation, a semistatic approach with frequent medium replacements was required to ensure a stable medium concentration. Laurate was also rapidly metabolized by H. azteca. A large proportion of the total radioactivity measured in the amphipod tissue was not extractable, suggesting that mineralized laurate was accumulated in the calcified exoskeleton of H. azteca. This was confirmed in a further study using carbonate [14 C]. A lipid-normalized (5.0%) Hyalella BCF of 8.9 was calculated for laurate, measured as free fatty acids. The results of the bioconcentration studies with H. azteca confirm the low bioaccumulation potential of the test item previously observed in fish. However, more organic ionic compounds with various properties need to be tested to assess whether a general correlation between fish and Hyalella BCF data exists. Environ Toxicol Chem 2020;39:310-322. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC.

中文翻译：

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14 C-标记的月桂酸盐在淡水两栖类透明藻中的生物浓缩，代谢和空间分布。

水中生物富集的监管评估通常基于鱼类流过测试得出的生物富集因子（BCF）。这样的实验需要许多实验动物，并且既费时又费钱。最近有人提出了另一种使用两栖类透明质Hyalella azteca对有机中性化合物进行测试的方法，其结果表明BCF值与鱼类BCF数据具有很强的相关性。在本研究中，阐明了阿兹台克人中离子型化合物月桂酸酯的生物富集潜力。将1-14 C月桂酸酯的钠盐以流穿和半静态的方式施用于阿兹台克人。由于快速的生物降解，需要使用频繁更换培养基的半静态方法来确保稳定的培养基浓度。月桂酸酯也被阿兹台克人迅速代谢。在两栖动物组织中测得的总放射性中有很大一部分是不可提取的，这表明矿化的月桂酸酯积累在阿兹台克人钙化的外骨骼中。使用碳酸盐[14 C]进行的进一步研究证实了这一点。计算出月桂酸酯的脂质归一化（5.0％）Hyalella BCF为8.9，以游离脂肪酸测量。阿兹台克人生物富集研究的结果证实了先前在鱼类中观察到的测试项目的低生物蓄积潜力。但是，需要测试更多具有各种特性的有机离子化合物，以评估鱼类和Hyalella BCF数据之间是否存在一般相关性。Environ Toxicol Chem 2020； 39：310-322。©2019作者。Wiley Periodicals，Inc.代表SETAC出版的《环境毒理学和化学》。