bHLH115 transcription factor (TF) is a positive regulator of the Fe-deficiency and plays essential roles in the stress-related regulation network. In this study, orthologous bHLH115 genes in Arabidopsis, tomato, rice, and maize were analyzed using in silico methods. All bHLH115 proteins contained PF00010 (HLH: Helix-loop-helix DNA-binding domain) domain structure and their sub-cellular localizations were predicted as nucleus. The bHLH115 orthologues in monocots and dicots clearly diverged from each other. The expression analyses revealed that orthologous genes of bHLH115 in queried species were highly expressed in seed parts, leaf, stem, and flower parts. The bHLH115 genes were co-expressed with genes in plant defense system, and with genes involving in biotic and abiotic stress responses. In terms of protein structures, OsbHLH115 and ZmbHLH115, and AtbHLH115 and SlbHLH115 had the highest protein structure similarities. In addition, bHLH115 proteins have bZIP, bHLH and MYB transcription factor binding sites strengthens their engagement in various metabolic ways. Molecular docking analyses showed the different binding sites based on plant species, suggesting functional flexibilities of bHLH115 gene.

中文翻译：

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铁稳态中的关键基因bHLH115：拟南芥，番茄，水稻和玉米中的综合生物信息学分析。

bHLH115转录因子（TF）是铁缺乏症的积极调节者，并在与压力有关的调节网络中发挥重要作用。在这项研究中，使用计算机模拟方法分析了拟南芥，番茄，水稻和玉米中的直系同源bHLH115基因。所有bHLH115蛋白均包含PF00010（HLH：螺旋-环-螺旋DNA结合结构域）结构域，其亚细胞定位被预测为细胞核。单子叶植物和双子叶植物中的bHLH115直向同源物显然彼此不同。表达分析表明，在所查询物种中，bHLH115的直系同源基因在种子部分，叶，茎和花部分中高表达。bHLH115基因与植物防御系统中的基因以及涉及生物和非生物胁迫响应的基因共表达。就蛋白质结构而言，OsbHLH115和ZmbHLH115 AtbHLH115和SlbHLH115具有最高的蛋白质结构相似性。此外，bHLH115蛋白具有bZIP，bHLH和MYB转录因子结合位点，可增强其以各种代谢方式的参与度。分子对接分析显示了基于植物种类的不同结合位点，表明了bHLH115基因的功能灵活性。