Superficial fascia has abundant preadipocytes capable of spontaneous and induced differentiation and is thought to be a novel origin of adipocytes. This study aimed to quantitatively evaluate the spatial distribution and correlation of adipocytes and mast cells in rat superficial fascia. Panoramic images were obtained from whole-mounted fascia stained by toluidine blue. Adipocytes increased gradually in superficial fascia of growing rats. Abundant mast cells, with the degranulation and exocytosis of abundant secretory granules, appeared in fascia where partially differentiating adipocytes and mature adipocytes occurred. Quantitative histological analysis by variance-mean ratio and Morisita index of dispersion indicated that both mast cells and adipocytes in fascia were distributed individually in cluster, but not random or uniform. Spearman's correlation coefficient revealed that the spatial cluster distributions of mast cells and adipocytes positively correlated with each other and correlated with increased number and size of adipocytes and adipogenic areas in fascia. Morphometry analysis indicated the strong correlation between fascial adipocytes and mast cells during the periods of rat growth. The correlation coefficient increased significantly at 8 weeks compared to 4 weeks, consistent with the increasing trend of the number and size of fascia adipocytes in growing rats. This finding provided the first quantitative histological analysis for the spatial distribution and correlation of fascia adipocytes and mast cells, which could be the histocytological basis for further exploring spatial and functional interactions between fascial mast cells and adipocytes. Also, the present data were informative for the research on physiologies and pathologies of fascia and fascia-related connective tissues.

中文翻译：

---

大鼠浅筋膜中脂肪细胞和肥大细胞的空间分布及相关性。

浅筋膜具有丰富的能够自发和诱导分化的前脂肪细胞，被认为是脂肪细胞的新来源。这项研究旨在定量评估大鼠浅筋膜中脂肪细胞和肥大细胞的空间分布和相关性。从用甲苯胺蓝染色的整个安装的筋膜获得全景图像。在成年大鼠的浅筋膜中，脂肪细胞逐渐增加。筋膜中出现大量肥大细胞，具有大量分泌性颗粒的脱颗粒和胞吐作用，在筋膜中出现部分分化的脂肪细胞和成熟的脂肪细胞。通过方差平均比和Morisita分散指数对组织学进行定量分析表明，筋膜中的肥大细胞和脂肪细胞均呈簇状分布，而不是随机或均匀分布。矛兵 相关系数表明，肥大细胞和脂肪细胞的空间簇分布正相关，并且与筋膜中脂肪细胞和脂肪形成区域的数量和大小增加相关。形态计量学分析表明在大鼠生长期间筋膜脂肪细胞和肥大细胞之间有很强的相关性。在8周时，相关系数显着增加，而在4周时，相关系数显着增加，这与成年大鼠筋膜脂肪细胞的数量和大小的增加趋势一致。这一发现为筋膜脂肪细胞与肥大细胞的空间分布和相关性提供了首次定量组织学分析，这可能是进一步探索筋膜肥大细胞与脂肪细胞之间空间和功能相互作用的组织学基础。还，