Function and dysfunction of endothelial cells are regulated by a multitude of factors. Endothelial cell research often requires in vitro cell culture experiments. Hence, various culture media specifically designed to promote endothelial cell growth are available. These strikingly differ in their composition: complex media contain endothelial cell growth supplement (ECGS), an extract produced of bovine brain with undefined amounts of biologically active compounds, whilst defined media contain selected growth factors in defined concentrations. We here compared the effect of seven purchasable endothelial cell culture media on colony outgrowth, proliferation, viability, in vitro angiogenesis and phenotype of mature primary human endothelial cells using feto-placental endothelial cells isolated from chorionic arteries (fpEC). The effect of media on colony outgrowth was additionally tested on umbilical cord blood-derived endothelial progenitor cells (ECFCs). Outgrowth, purity, proliferation and viability differed between media. Outgrowth of fpEC and ECFCs was best in a defined medium containing EGF, FGF2 and VEGF. By contrast, established fpEC isolations proliferated best in complex media containing ECGS, heparin and ascorbic acid. Also viability of cells was higher in complex media. In vitro angiogenesis was most intense in a defined medium containing the highest number of individual growth factors. FACS analysis of surface markers for endothelial cell subtypes revealed that endothelial phenotype of fpEC was unaffected by media composition. Our data demonstrate the fundamental effect of endothelial cell culture media on primary cell isolation success and behaviour. Whether the composition of supplements is suitable also for individual experiments needs to be tested specifically.

中文翻译：

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原代人内皮细胞在不同可购买的内皮培养基中的生长、增殖、活力、血管生成和表型：明智地喂养。

内皮细胞的功能和功能障碍受多种因素的调节。内皮细胞研究通常需要体外细胞培养实验。因此，可以使用专门设计用于促进内皮细胞生长的各种培养基。它们的成分明显不同：复合培养基含有内皮细胞生长补充剂 (ECGS)，这是一种从牛脑中提取的提取物，含有不确定数量的生物活性化合物，而成分明确的培养基则含有特定浓度的选定生长因子。我们在这里使用从绒毛膜动脉 (fpEC) 分离的胎儿胎盘内皮细胞比较了七种可购买的内皮细胞培养基对集落生长、增殖、活力、体外血管生成和成熟原代人内皮细胞表型的影响。在脐带血衍生的内皮祖细胞 (ECFC) 上另外测试了培养基对集落生长的影响。培养基之间的生长、纯度、增殖和活力不同。fpEC 和 ECFCs 在含有 EGF、FGF2 和 VEGF 的成分确定的培养基中生长最好。相比之下，已建立的 fpEC 分离物在含有 ECGS、肝素和抗坏血酸的复杂培养基中增殖最好。细胞在复杂培养基中的活力也更高。体外血管生成在含有最多个体生长因子的成分确定的培养基中最为强烈。内皮细胞亚型表面标志物的 FACS 分析表明，fpEC 的内皮表型不受培养基成分的影响。我们的数据证明了内皮细胞培养基对原代细胞分离成功和行为的基本影响。