Although neutrophils are considered a histologic hallmark of psoriasis, their pathophysiologic role in psoriasis remains unclear. We characterized the effects of neutrophil depletion via injection of monoclonal antibody 1A8 on the development of imiquimod (IMQ)-induced psoriatic lesions in a murine model. Lesions were followed with photographs and histologic analysis, revealing reduced psoriasiform scale and epidermal hyperplasia in neutrophil-depleted. ELISA and flow cytometry were used to determine relative levels of cytokines and immune cells. Compared to controls, IMQ-treated neutropenic mice had significantly lower levels of macrophages in tissue samples (P < .05) and displayed significantly lower numbers of CD4+ T-cells (P < .05). Neutropenic animals exhibited lower levels of TNF-α, IFN-γ, and IL-1β than controls (P < .05). These results show that neutropenia reduces the development of psoriasiform skin lesions and substantially decreases infiltration of pro-inflammatory cytokines and immune cells to IMQ-induced cutaneous lesions, suggesting an active role of neutrophils in maintaining inflammation in psoriasis.

中文翻译：

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中性粒细胞的生物耗竭减弱了牛皮癣小鼠模型中的促炎性标志物和牛皮癣表型的发展。

尽管嗜中性粒细胞被认为是牛皮癣的组织学标志，但尚不清楚它们在牛皮癣中的病理生理作用。我们表征了通过注射单克隆抗体1A8对中性白细胞耗竭对咪喹莫特（IMQ）诱导的鼠类银屑病皮损的发展的影响。病变后进行照片和组织学分析，发现嗜中性白血球减少的牛皮癣规模减少和表皮增生。ELISA和流式细胞仪用于确定细胞因子和免疫细胞的相对水平。与对照组相比，IMQ处理的嗜中性白血球减少症小鼠组织样品中的巨噬细胞水平显着降低（P <.05），而CD4 + T细胞的数量则显着降低（P <.05）。中性粒细胞减少的动物的TNF-α，IFN-γ和IL-1β的水平低于对照组（P <.05）。