Environmentally responsive nanomaterials have been developed for drug delivery applications, in an effort to target and accumulate therapeutic agents at sites of disease. Within a biological system, these nanomaterials will experience diverse conditions which encompass a variety of solute identities and concentrations. In this study, we developed a new quartz crystal microbalance with dissipation (QCM‐D) assay, which enabled the quantitative analysis of nanogel swelling, protein adsorption, and biodegradation in a single experiment. As a proof of concept, we employed this assay to characterize non‐degradable and biodegradable poly(acrylamide‐co‐methacrylic acid) nanogels. We compared the QCM‐D results to those obtained by dynamic light scattering to highlight the advantages and limitations of each method. We detailed our protocol development and practical recommendations, and hope that this study will serve as a guide for others to design application‐specific QCM‐D assays within the nanomedicine domain. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48655.

中文翻译：

---

用于量化智能纳米凝胶的溶胀、生物降解和蛋白质吸附的 QCM-D 测定

已开发出用于药物递送应用的环境响应纳米材料，以在疾病部位靶向和积累治疗剂。在生物系统中，这些纳米材料将经历包括各种溶质特性和浓度在内的各种条件。在这项研究中，我们开发了一种新的石英晶体微天平与耗散 (QCM-D) 分析，它能够在单个实验中对纳米凝胶溶胀、蛋白质吸附和生物降解进行定量分析。作为概念证明，我们使用该测定来表征不可降解和可生物降解的聚（丙烯酰胺-co-甲基丙烯酸）纳米凝胶。我们将 QCM-D 结果与通过动态光散射获得的结果进行了比较，以突出每种方法的优点和局限性。我们详细介绍了我们的协议开发和实用建议，并希望这项研究将作为其他人在纳米医学领域设计特定应用 QCM-D 检测的指南。© 2019 Wiley Periodicals, Inc. J. Appl。聚合物。科学。2020，137，48655。