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Evaluating the cytocompatibility and differentiation of bone progenitors on electrospun zein scaffolds.
Journal of Tissue Engineering and Regenerative Medicine ( IF 3.3 ) Pub Date : 2019-11-15 , DOI: 10.1002/term.2984
Jessica Cardenas Turner 1 , George Collins 1 , Elizabeth A Blaber 2 , Eduardo A C Almeida 2 , Treena L Arinzeh 1
Affiliation  

Bone fractures often result in complications that require surgical intervention to promote fracture healing. Tissue engineering seeks to alleviate the need for autologous bone grafting by utilizing scaffolds that can promote bone fracture healing. Plant-derived materials are desirable biomaterials because of their biodegradability, availability, and low immunogenicity. Among various plant-derived proteins, zein, which is a corn protein, has shown promise for bone repair. However, when processed, zein is often blended with synthetic materials to improve mechanical properties and overall hydrolytic stability. In this study, pure zein was electrospun to create fibrous scaffolds and cross-linked with trimethylolpropane triglycidyl ether to improve hydrolytic stability. Scaffolds were characterized and evaluated in vitro for promoting the osteogenic differentiation of MC3T3-E1 cells, which are bone progenitor cells. Cross-linked zein scaffolds retained their uniform fiber morphologies after hydration. MC3T3-E1 cells grew and differentiated on the zein scaffolds even in the absence of induction factors, as demonstrated by increased alkaline phosphatase activity, mineralization, and early upregulation of Runx2 gene expression, a transcription factor associated with osteoblast differentiation. These studies demonstrate that stable, zein fibrous scaffolds could have potential for use in bone repair applications.

中文翻译:

评价电纺玉米蛋白支架上骨祖细胞的细胞相容性和分化。

骨骨折通常会导致并发症,需要手术干预以促进骨折愈合。组织工程学试图通过利用可促进骨折愈合的支架来减轻对自体骨移植的需求。植物来源的材料是理想的生物材料,因为它们具有生物降解性,可利用性和低免疫原性。在多种植物来源的蛋白质中,玉米蛋白玉米醇溶蛋白显示出有望修复骨的潜力。然而,在加工时,玉米蛋白通常与合成材料混合以改善机械性能和总体水解稳定性。在这项研究中,纯玉米醇溶蛋白被静电纺丝以形成纤维状支架,并与三羟甲基丙烷三缩水甘油醚交联以提高水解稳定性。对支架进行表征和体外评估以促进MC3T3-E1细胞(其为骨祖细胞)的成骨分化。交联的玉米醇溶蛋白支架在水合后保持其均匀的纤维形态。即使缺乏诱导因子,MC3T3-E1细胞也可以在玉米醇溶蛋白支架上生长和分化,这表现为碱性磷酸酶活性增强,矿化和Runx2基因表达的早期上调,Runx2基因表达是与成骨细胞分化相关的转录因子。这些研究表明,稳定的玉米醇溶蛋白纤维支架可能具有用于骨修复应用的潜力。即使缺乏诱导因子,MC3T3-E1细胞也可以在玉米醇溶蛋白支架上生长和分化,这表现为碱性磷酸酶活性增强,矿化和Runx2基因表达的早期上调,Runx2基因表达是与成骨细胞分化相关的转录因子。这些研究表明,稳定的玉米醇溶蛋白纤维支架可能具有用于骨修复应用的潜力。即使缺乏诱导因子,MC3T3-E1细胞也可以在玉米醇溶蛋白支架上生长和分化,这表现为碱性磷酸酶活性增强,矿化和Runx2基因表达的早期上调,Runx2基因表达是与成骨细胞分化相关的转录因子。这些研究表明,稳定的玉米醇溶蛋白纤维支架可能具有用于骨修复应用的潜力。
更新日期:2019-11-17
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